Diaporthe diversity and pathogenicity revealed from a broad survey of grapevine diseases in Europe

被引：147

作者：

Guarnaccia, V. ^{[1
]}

Groenewald, J. Z. ^{[1
]}

Woodhall, J. ^{[2
,3
]}

Armengol, J. ^{[4
]}

Cinelli, T. ^{[5
]}

Eichmeier, A. ^{[6
]}

Ezra, D. ^{[7
]}

Fontaine, F. ^{[8
]}

Gramaje, D. ^{[9
]}

Gutierrez-Aguirregabiria, A. ^{[2
,10
]}

Kaliterna, J. ^{[11
]}

Kiss, L. ^{[12
,13
]}

Larignon, P. ^{[14
]}

Luque, J. ^{[15
]}

Mugnai, L. ^{[5
]}

Naor, V. ^{[16
]}

Raposo, R. ^{[17
]}

Sandor, E. ^{[18
]}

Vaczy, K. Z. ^{[19
]}

Crous, P. W. ^{[1
,20
,21
]}

机构：

[1] Westerdijk Fungal Biodivers Inst, Uppsalalaan 8, NL-3584 CT Utrecht, Netherlands

[2] Fera, York YO41 1LZ, N Yorkshire, England

[3] Univ Idaho, Parma Res & Extens Ctr, Parma, ID USA

[4] Univ Politecn Valencia, Inst Agroforestal Mediterraneo, Camino Vera S-N, E-46022 Valencia, Spain

[5] Univ Firenze, Sez Patol Vegetale & Entomol, Dipartimento Sci Prod Agroalimentari & Ambiente D, Piazzale Cascine 28, I-50144 Florence, Italy

[6] Mendel Univ Brno, Fac Hort, Mendeleum, Dept Genet, Valticka 337, Lednice 69144, Czech Republic

[7] ARO Volcani Ctr, 68 HaMacabim Rd, IL-7505101 Rishon Leziyyon, Israel

[8] Univ Reims, SFR Condorcet, URVVC EA 4707, Lab Stress Def & Reprod Plantes, BP 1039, F-51687 Reims 2, France

[9] Univ La Rioja, CSIC, Inst Ciencias Vid & Vino, Gobierno De La Rioja 26007, Logrono, Spain

[10] Univ Southampton, Fac Nat & Environm Sci, Highfield Campus, Southampton SO17 1BJ, Hants, England

[11] Univ Zagreb, Dept Plant Pathol, Fac Agr, Svetosimunska 25, Zagreb 10000, Croatia

[12] Univ Southern Queensland, Ctr Crop Hlth, Toowoomba, Qld 4350, Australia

[13] Hungarian Acad Sci, Ctr Agr Res, H-2462 Martonvasar, Hungary

[14] Inst Francais Vigne & Vin, Pole Rhone Mediterranee, 7 Ave Cazeaux, F-30230 Rodilhan, France

[15] IRTA Ctr Cabrils, Carretera Cabrils Km 2, Cabrils 08348, Spain

[16] Shamir Res Inst, Katsrin, Israel

[17] INIA CIFOR, C Coruna Km 7-5, Madrid 28040, Spain

[18] Univ Debrecen, Inst Food Sci, Boszormenyi Ut 138, H-4032 Debrecen, Hungary

[19] Eszterhazy Karoly Univ, Ctr Res & Dev, H-3300 Eger, Hungary

[20] Wageningen Univ & Res Ctr WUR, Lab Phytopathol, Droevendaalsesteeg 1, NL-6708 PB Wageningen, Netherlands

[21] Univ Pretoria, Dept Microbiol & Plant Pathol, FABI, P Bag X20, ZA-0028 Pretoria, South Africa

来源：

PERSOONIA | 2018年 / 40卷

基金：

欧盟地平线“2020”;

关键词：

canker; multi-locus sequence typing; pathogenicity; Vitis; ENDOPHYTIC FUNGUS PHOMOPSIS; 1ST REPORT; CITRUS DISEASES; GENUS DIAPORTHE; PRIMER SETS; SP NOV; DIEBACK; REASSESSMENT; PHYLOGENY; CANKERS;

D O I：

10.3767/persoonia.2018.40.06

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Species of Diaporthe are considered important plant pathogens, saprobes, and endophytes on a wide range of plant hosts. Several species are well-known on grapevines, either as agents of pre-or post-harvest infections, including Phomopsis cane and leaf spot, cane bleaching, swelling arm and trunk cankers. In this study we explore the occurrence, diversity and pathogenicity of Diaporthe spp. associated with Vitis vinifera in major grape production areas of Europe and Israel, focusing on nurseries and vineyards. Surveys were conducted in Croatia, Czech Republic, France, Hungary, Israel, Italy, Spain and the UK. A total of 175 Diaporthe strains were isolated from asymptomatic and symptomatic shoots, branches and trunks. A multi-locus phylogeny was established based on five genomic loci (ITS, tef1, cal, his3 and tub2), and the morphological characters of the isolates were determined. Preliminary pathogenicity tests were performed on green grapevine shoots with representative isolates. The most commonly isolated species were D. eres and D. ampelina. Four new Diaporthe species described here as D. bohemiae, D. celeris, D. hispaniae and D. hungariae were found associated with affected vines. Pathogenicity tests revealed D. baccae, D. celeris, D. hispaniae and D. hungariae as pathogens of grapevines. No symptoms were caused by D. bohemiae. This study represents the first report of D. ambigua and D. baccae on grapevines in Europe. The present study improves our understanding of the species associated with several disease symptoms on V. vinifera plants, and provides useful information for effective disease management.

引用

页码：135 / 153

页数：19

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