Detection of Brucella melitensis in Milk of Hair Goat (Capra hircus) by Polymerase Chain Reaction (PCR)

被引：0

作者：

Keskin, Dilek ^{[1
]}

Atay, Okan ^{[1
]}

Kirkan, Suekrue ^{[2
]}

Gokdal, Ozdal ^{[1
]}

Tekbiyik, Serten ^{[2
]}

Kaya, Osman ^{[2
]}

Eren, Vadullah ^{[1
]}

机构：

[1] Adnan Menderes Univ, Cine Vocat Sch, Cine, Aydin, Turkey

[2] Adnan Menderes Univ, Fac Vet Med, Aydin, Turkey

来源：

KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI | 2009年 / 15卷 / 02期

关键词：

Brucella melitensis; IS711; PCR; Hair Goat; Milk; SINGLE-STEP PCR; ABORTUS; DIAGNOSIS; CATTLE; BLOOD; SHEEP; SPP;

D O I：

暂无

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

The aim of this study was to investigate to B. melitensis in Hair Goat in the Cine district of Aydin province. B. melitensis, is a significant problem for public and animal health in Turkey. In this study, 222 milk samples from 116 goats were collected from 10 flocks in the villages of Kavsit, Mutaflar, Tatarmemisler and Elderesi. Hair goat of 2-7 years of age that no history of vaccination against brucellosis. in this study, oligonucleotide primers specific for IS711, B. melitensis and B. abortus were used. After DNA extraction from the milk samples, PCR was used to detect B. melitensis and 50 positive results (22.5%) of milk samples of Hair Goat produced a 731 bp PCR band specific for B. melitensis.

引用

页码：255 / 259

页数：5

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