Genetic reprogramming of somatic cells into neuroblasts through a co-induction of the doublecortin gene along the Yamanaka factors: A promising approach to model neuroregenerative disorders

被引：2

作者：

Kandasamy, Mahesh ^{[1
,2
,5
]}

Yesudhas, Ajisha ^{[1
]}

Abirami, G. P. Poornimai ^{[2
]}

Radhakrishnan, Risna Kanjirassery ^{[1
]}

Roshan, Syed Aasish ^{[3
]}

Johnson, Esther ^{[1
]}

Ravichandran, Vijaya Roobini ^{[1
]}

Biswas, Abir ^{[3
]}

Shanmugaapriya, Sellathamby ^{[4
]}

Anusuyadevi, Muthuswamy ^{[2
,3
]}

Aigner, Ludwig ^{[6
,7
]}

机构：

[1] Bharathidasan Univ, Sch Life Sci, Dept Anim Sci, Lab Stem Cells & Neuroregenerat, Tiruchirappalli, Tamil Nadu, India

[2] Bharathidasan Univ, Sch Life Sci, Tiruchirappalli, Tamil Nadu, India

[3] Bharathidasan Univ, Sch Life Sci, Dept Biochem, Mol Gerontol Lab, Tiruchirappalli, Tamil Nadu, India

[4] Bharathidasan Univ, Dept Biomed Sci, Tiruchirappalli, Tamil Nadu, India

[5] Univ Grants Commiss, UGC FRP, New Delhi, India

[6] Paracelsus Med Univ, Inst Mol Regenerat Med, Strubergasse 21, A-5020 Salzburg, Austria

[7] Salzburg Paracelsus Med Univ, Spinal Cord Injury & Tissue Regenerat Ctr, Salzburg, Austria

来源：

MEDICAL HYPOTHESES | 2019年 / 127卷

关键词：

iPSCs; Doublecortin; Neuroblasts; Genetic reprogramming; Neurodegenerative disorders; PLURIPOTENT STEM-CELLS; ADULT HIPPOCAMPAL NEUROGENESIS; ECTOPIC MIGRATION; HUMAN BRAIN; PSA-NCAM; DISEASE; DIFFERENTIATION; NEURONS; EXPRESSION; INDUCTION;

D O I：

10.1016/j.mehy.2019.04.006

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Neural stem cell (NSC) mediated adult neurogenesis represents the regenerative plasticity of the brain. The functionality of the neurogenic process appears to be operated by neuroblasts, the multipotent immature neuronal population of the adult brain. While neuroblasts have been realized to play a major role in synaptic remodeling and immunogenicity, neurodegenerative disorders have been characterized by failure in the terminal differentiation, maturation, integration and survival of newborn neuroblasts. Advancement in understanding the impaired neuroregenerative process along the neuropathological conditions has currently been limited by lack of an appropriate experimental model of neuroblasts. The genetic reprogramming of somatic cells into pluripotent state offers a potential strategy for the experimental modeling of brain disorders. Thus, the induced pluripotent stem cell (iPSC) based direct reprogramming of somatic cells into neuroblasts would represent a potential tool to understand the regenerative biology of the adult brain. Therefore, this concise article discusses the significance of IPSCs, the functional roles of neuroblasts in the adult brain and provides a research hypothesis for the direct reprogramming of somatic cells into neuroblasts through the co-induction of a potential proneurogenic marker, the doublecortin (DCX) gene along with the Yamanaka factors. The proposed cellular model of adult neurogenesis may provide us with further insights into neuropathogenesis of many neurodegenerative disorders and will provide a potential experimental platform for diagnostic, drug discovery and regenerative therapeutic strategies.

引用

页码：105 / 111

页数：7

共 110 条

[1] Preconditioning and Cellular Engineering to Increase the Survival of Transplanted Neural Stem Cells for Motor Neuron Disease Therapy [J].