Deadlock, a novel protein of Drosophila, is required for germline maintenance, fusome morphogenesis and axial patterning in oogenesis and associates with centrosomes in the early embryo

被引:17
作者
Wehr, Kristina [1 ]
Swan, Andrew [1 ]
Schupbach, Trudi [1 ]
机构
[1] Princeton Univ, Howard Hughes Med Inst, Dept Mol Biol, Princeton, NJ 08544 USA
关键词
Drosophila; oogenesis; fusome; germline stem cell; microtubules; spermatogenesis;
D O I
10.1016/j.ydbio.2006.03.002
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The deadlock gene is required for a number of key developmental events in Drosophila oogenesis. Females homozygous for mutations in the deadlock gene lay few eggs and those exhibit severe patterning defects along both the anterior-posterior and dorsal-ventral axis. In this study, we analyzed eggs and ovaries from deadlock mutants and determined that deadlock is required for germline maintenance, stability of mitotic spindles, localization of patterning determinants, oocyte growth and fusome biogenesis in males and females. Deadlock encodes a novel protein which colocalizes with the oocyte nucleus at midstages of oogenesis and with the centrosomes of early embryos. Our genetic and immunohistological experiments point to a role for Deadlock in microtubule function during oogenesis. (c) 2006 Elsevier Inc. All rights reserved.
引用
收藏
页码:406 / 417
页数:12
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