A single P450 allele associated with insecticide resistance in Drosophila

被引：666

作者：

Daborn, PJ

Yen, JL

Bogwitz, MR

Le Goff, G

Feil, E

Jeffers, S

Tijet, N

Perry, T

Heckel, D

Batterham, P

Feyereisen, R

Wilson, TG

ffrench-Constant, RH ^{[1
]}

机构：

[1] Univ Bath, Dept Biol & Biochem, Bath BA2 7AY, Avon, England

[2] Univ Melbourne, Ctr Environm Stress & Adaptat Res, Dept Genet, Parkville, Vic 3052, Australia

[3] Colorado State Univ, Dept Biol, Ft Collins, CO 80523 USA

[4] Univ Arizona, Dept Entomol, Tucson, AZ 85721 USA

[5] INRA, Ctr Rech Antibes, F-06560 Valbonne, France

来源：

SCIENCE | 2002年 / 297卷 / 5590期

关键词：

D O I：

10.1126/science.1074170

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Insecticide resistance is one of the most widespread genetic changes caused by human activity, but we still understand little about the origins and spread of resistant alleles in global populations of insects. Here, via microarray analysis of all P450s in Drosophila melanogaster, we show that DDT-R, a gene conferring resistance to DDT, is associated with overtranscription of a single cytochrome P450 gene, Cyp6g1. Transgenic analysis of Cyp6g1 shows that overtranscription of this gene alone is both necessary and sufficient for resistance. Resistance and up-regulation in Drosophila populations are associated with a single Cyp6g1 allele that has spread globally. This allele is characterized by the insertion of an Accord transposable element into the 5' end of the Cyp6g1 gene.

引用

页码：2253 / 2256

页数：5

共 30 条

[1] Cytochrome P450 monooxygenases and insecticide resistance in insects [J].