(-)-Epigallocatechin-3-gallate suppresses cigarette smoke-induced inflammation in human cardiomyocytes via ROS-mediated MAPK and NF-κB pathways

被引:46
作者
Liang, Yingmin [1 ]
Ip, Mary Sau Man [1 ]
Mak, Judith Choi Wo [1 ,2 ]
机构
[1] Univ Hong Kong, Li Ka Shing Fac Med, Dept Med, Pokfulam, 21 Sassoon Rd, Hong Kong, Peoples R China
[2] Univ Hong Kong, Li Ka Shing Fac Med, Dept Pharmacol & Pharm, Pokfulam, 21 Sassoon Rd, Hong Kong, Peoples R China
关键词
Cigarette smoke; (-)-epigallocatechin-3-gallate; Oxidative stress; Inflammation; Cardiomyocytes; GREEN TEA CONSUMPTION; OXIDATIVE STRESS; CARDIOVASCULAR-DISEASE; PROTECTS CARDIOMYOCYTES; ENDOTHELIAL-CELLS; GENE-EXPRESSION; TNF-ALPHA; ACTIVATION; MECHANISMS; EGCG;
D O I
10.1016/j.phymed.2018.11.028
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Background: Cigarette smoking is the leading cause for the initiation and development of cardiovascular disease (CVD). Oxidative stress and inflammatory responses play important roles in the pathophysiological processes of smoking-induced cardiac injury. (-)-epigallocatechin-3-gallate (EGCG), the most abundant catechin in green tea, which is made from Camellia sinensis leaves, has been reported to possess potent anti-oxidant property. Purpose: This study aims to investigate whether the antioxidant EGCG could alleviate cigarette smoke medium (CSM)-induced inflammation in human AC16 cardiomyocytes in vitro. Methods: Human AC16 cardiomyocytes were pre-treated with EGCG, N-acetyl-L-cysteine (NAC), or specific inhibitors for 30 min before 4% CSM was added. Supernatant was collected for determination of interleukin (IL)-8 by ELISA and cells were collected for flow cytometry, biochemical assays and Western blot analysis. Results: EGCG treatment significantly attenuated CSM-induced oxidative stress as evidenced by reducing intracellular and mitochondrial reactive oxygen species (ROS) generations and preventing antioxidant depletion. EGCG treatment reduced CSM-induced inflammatory chemokine interleukin (IL)-8 productions in the supernatant via the inhibition of ERK1/2, p38 MAPK and NF-kappa B pathways. EGCG treatment further inhibited CSM-induced cell apoptosis. Conclusion: Taken together, EGCG protected against CSM-induced inflammation and cell apoptosis by attenuating oxidative stress via inhibiting ERK1/2, p38 MAPK, and NF-kappa B activation in AC16 cardiomyocytes. These findings suggest that EGCG with its antioxidant, anti-inflammatory and anti-apoptotic properties may act as a promising cardioprotective agent against ROS-mediated cardiac injury.
引用
收藏
页数:10
相关论文
共 51 条
[1]   (-)-Epigallocatechin-gallate (EGCG) stabilize the mitochondrial enzymes and inhibits the apoptosis in cigarette smoke-induced myocardial dysfunction in rats [J].
Adikesavan, Gokulakrishnan ;
Vinayagam, Magendira Mani ;
Abdulrahman, Liyakath Ali ;
Chinnasamy, Thirunavukkarasu .
MOLECULAR BIOLOGY REPORTS, 2013, 40 (12) :6533-6545
[2]   Epigallocatechin-3-O-gallate inhibits TNFα-induced monocyte chemotactic protein-1 production from vascular endothelial cells [J].
Ahn, Hee Yul ;
Xu, Yi ;
Davidge, Sandra T. .
LIFE SCIENCES, 2008, 82 (17-18) :964-968
[3]   The pathophysiology of cigarette C-V smoking and cardiovascular disease - An update [J].
Ambrose, JA ;
Barua, RS .
JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY, 2004, 43 (10) :1731-1737
[4]   Interleukin 8 and cardiovascular disease [J].
Apostolakis, Stavros ;
Vogiatzi, Konstantina ;
Amanatidou, Virginia ;
Spandidos, Demetrios A. .
CARDIOVASCULAR RESEARCH, 2009, 84 (03) :353-360
[5]   NF-κB, Inflammation, and Metabolic Disease [J].
Baker, Rebecca G. ;
Hayden, Matthew S. ;
Ghosh, Sankar .
CELL METABOLISM, 2011, 13 (01) :11-22
[6]   Mechanisms of Coronary Thrombosis in Cigarette Smoke Exposure [J].
Barua, Rajat S. ;
Ambrose, John A. .
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 2013, 33 (07) :1460-1467
[7]  
Bernhard D, 2007, CURR MED CHEM, V14, P1703
[8]   CENTRAL OF I-KAPPA-B-ALPHA PROTEOLYSIS BY SITE-SPECIFIC, SIGNAL-INDUCED PHOSPHORYLATION [J].
BROWN, K ;
GERSTBERGER, S ;
CARLSON, L ;
FRANZOSO, G ;
SIEBENLIST, U .
SCIENCE, 1995, 267 (5203) :1485-1488
[9]   Adverse Effects of Cigarette Smoke and Induction of Oxidative Stress in Cardiomyocytes and Vascular Endothelium [J].
Carver, Anabel Varela ;
Parker, Howard ;
Kleinert, Christina ;
Rimoldi, Ornella .
CURRENT PHARMACEUTICAL DESIGN, 2010, 16 (23) :2551-2558
[10]   A green tea-derived polyphenol, epigallocatechin-3-gallate, inhibits IκB kinase activation and IL-8 gene expression in respiratory epithelium [J].
Chen, PC ;
Wheeler, DS ;
Malhotra, V ;
Odoms, K ;
Denenberg, AG ;
Wong, HR .
INFLAMMATION, 2002, 26 (05) :233-241