Imaging the cell wall of living single yeast cells using surface-enhanced Raman spectroscopy

被引：61

作者：

Sujith, Athiyanathil ^{[1
]}

Itoh, Tamitake ^{[1
]}

Abe, Hiroko ^{[1
]}

Yoshida, Ken-ichi ^{[1
]}

Kiran, Manikantan S. ^{[1
]}

Biju, Vasudevanpillai ^{[1
]}

Ishikawa, Misturu ^{[1
]}

机构：

[1] Natl Inst Adv Ind Sci & Technol, Hlth Technol Res Ctr, Kagawa 7610395, Japan

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2009年 / 394卷 / 07期

关键词：

Surface-enhanced Raman spectroscopy; Living cell analysis; Raman spectroscopic imaging; Yeast cell wall; Spectroscopy/Theory; Laser spectroscopy; Cell systems/Single cell analysis; SACCHAROMYCES-CEREVISIAE; GOLD NANOPARTICLES; SCATTERING; SILVER; PROTEINS; DNA; FLUCTUATIONS;

D O I：

10.1007/s00216-009-2883-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The surface of a living yeast cell (Saccharomyces cerevisiae strain W303-1A) has been labeled with silver (Ag) nanoparticles that can form nanoaggregates which have been shown to have surface-enhanced Raman scattering (SERS) activity. The cell wall of a single living yeast cell has been imaged by use of a Raman microspectroscope. The SERS spectra measured from different Ag nanoaggregates were found to be different. This can be explained on the basis of detailed spectral interpretation. The SERS spectral response originates from mannoproteins which cover the outermost regions of the yeast cell wall. Analysis of SERS spectra from the cell wall and the extracted mannoproteins from the yeast has been performed for the clarification of variation in SERS spectra.

引用

页码：1803 / 1809

页数：7

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