Graphene oxide-gold nanoparticles hybrids-based surface plasmon resonance for sensitive detection of microRNA

被引：123

作者：

Wang, Qing ^{[1
]}

Li, Qing ^{[1
]}

Yang, Xiaohai ^{[1
]}

Wang, Kemin ^{[1
]}

Du, Shasha ^{[1
]}

Zhang, Hua ^{[1
]}

Nie, Yajie ^{[1
]}

机构：

[1] Hunan Univ, Key Lab Bio Nanotechnol & Mol Engn Hunan Prov, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Peoples R China

来源：

BIOSENSORS & BIOELECTRONICS | 2016年 / 77卷

基金：

中国国家自然科学基金; 对外科技合作项目（国际科技项目）;

关键词：

Surface plasmon resonance; MicroRNA; Graphene oxide-gold nanoparticles hybrids; LABEL-FREE DETECTION; SIGNAL AMPLIFICATION; ELECTROCHEMICAL BIOSENSOR; ULTRASENSITIVE DETECTION; MICROARRAY DETECTION; IMAGING MEASUREMENTS; DNA HYBRIDIZATION; HUMAN CANCERS; PROBES; ASSAY;

D O I：

10.1016/j.bios.2015.10.091

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

In this study, a simple and sensitive surface plasmon resonance (SPR) biosensor for miRNA detection was developed using graphene oxide-gold nanoparticles (GO-AuNPs) hybrids as signal amplification element. Taking advantage of the GO-AuNPs hybrids and their enhanced performance in SPR biosensors, the detection of miRNA was carried out in only two steps. Firstly, the thiolated capture DNA probe with a short complete complementary sequence was immobilized on the Au film surface to recognize the part sequence of target miRNA. Subsequently, the assistant DNA-linked GO-AuNPs hybrids were employed to bind the other section of the target. It was found that the developed SPR biosensor was able to achieve a detection limit as low as 1 fM. Moreover, the method showed excellent ability to discriminate differences among miRNA-200 family members. Notably, human miRNA from cancer cells could also be detected, and the results were in excellent agreement with the ones obtained using qRT-PCR. On the basis of these findings, we believe that this method has great potential for quantitative detection of miRNA in biomedical research and early clinical diagnostics. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：1001 / 1007

页数：7

共 45 条

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