Spirosoma aerolatum sp nov., isolated from a motor car air conditioning system

A Gram-stain-negative, yellow-pigmented bacterial strain, designated PR1012K(T), was isolated from a motor car evaporator core collected in Korea. Cells of the strain were facultatively anaerobic, non-spore-forming and rod-shaped. The strain grew at 10-40 degrees C (optimum, 25 degrees C), at pH 6.5-8.0 (optimum, pH 7.0-8.0) and in the presence of 0-1 % (w/v) NaCl. Phylogenetically, the strain was closely related to members of the genus Spirosoma (97.50-90.74 % 16S rRNA gene sequence similarities) and showed highest sequence similarity to Spirosoma panaciterrae DSM 21099(T) (97.50 %). Its predominant fatty acids included summed feature 3 (C-16 : 1 omega 7c and/or C-16 : 1 omega 6c), C-16 : 1 omega 5c, iso-C-15 : 0 and summed feature 4 (iso-C-17 : 1 I and/or anteiso B) and it had MK-7 as the major menaquinone. The polar lipids present included phosphatidylethanolamine, one unknown aminophospholipid, two unknown aminolipids and five unknown polar lipids. The DNA G + C content of this strain was 54 mol%. Based on phenotypic, genotypic and chemotaxonomic data, strain PR1012K(T) represents a novel species in the genus Spirosoma, for which the name Spirosoma aerolatum sp. nov. is proposed. The type strain is PR1012K(T) (=KACC 17939(T) =NBRC 110794(T)).