Induction of maturation and cytokine release of human dendritic cells by Helicobacter pylori

被引:96
作者
Kranzer, K
Eckhardt, A
Aigner, M
Knoll, G
Deml, L
Speth, C
Lehn, N
Rehli, M
Schneider-Brachert, W
机构
[1] Univ Regensburg, Inst Med Microbiol & Hyg, D-93053 Regensburg, Germany
[2] Univ Regensburg, Dept Hematol & Oncol, D-93053 Regensburg, Germany
[3] Univ Innsbruck, Inst Hyg & Social Med, A-6020 Innsbruck, Austria
关键词
D O I
10.1128/IAI.72.8.4416-4423.2004
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Helicobacter pylori causes a persistent infection in the human stomach, which can result in chronic gastritis and peptic ulcer disease. Despite an intensive proinflammatory response, the immune system is not able to clear the organism. However, the immune escape mechanisms of this common bacterium are not well understood. We investigated the interaction between H. pylori and human dendritic cells. Dendritic cells (DCs) are potent antigen-presenting cells and important mediators between the innate and acquired immune system. Stimulation of DCs with different concentrations of H. pylori for 8, 24, 48, and 72 h resulted in dose-dependent interleukin-6 (IL-6), IL-8, IL-10 and IL-12 production. Lipopolysaccharide (LPS) from Escherichia coli, a known DC maturation agent, was used as a positive control. The cytokine release after stimulation with LPS was comparable to that induced by H. pylori except for IL-12. After LPS stimulation IL-12 was only moderately released compared to the large amounts of IL-12 induced by H. pylori. We further investigated the potential of H. pylori to induce maturation of DCs. Fluorescence-activated cell sorting analysis of cell surface expression of maturation marker molecules such as CD80, CD83, CD86, and HLA-DR revealed equal upregulation after stimulation with H. pylori or LPS. We found no significant differences between H. pylori seropositive and seronegative donors of DCs with regard to cytokine release and upregulation of surface molecules. These data clearly demonstrate that H. pylori induces a strong activation and maturation of human immature DCs.
引用
收藏
页码:4416 / 4423
页数:8
相关论文
共 62 条
  • [1] Disruption of the epithelial apical-junctional complex by Helicobacter pylori CagA
    Amieva, MR
    Vogelmann, R
    Covacci, A
    Tompkins, LS
    Nelson, WJ
    Falkow, S
    [J]. SCIENCE, 2003, 300 (5624) : 1430 - 1434
  • [2] Anti-CagA immunoglobulin G responses correlate with interleukin-8 induction in human gastric mucosal biopsy culture
    Ando, T
    Perez-Perez, GI
    Kusugami, K
    Ohsuga, M
    Bloch, KC
    Blaser, MJ
    [J]. CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY, 2000, 7 (05) : 803 - 809
  • [3] Cutting edge: Carbohydrate profiling identifies new pathogens that interact with dendritic cell-specific ICAM-3-grabbing nonintegrin on dendritic cells
    Appelmelk, BJ
    van Die, I
    van Vliet, SJ
    Vandenbroucke-Grauls, CMJE
    Geijtenbeek, TBH
    van Kooyk, Y
    [J]. JOURNAL OF IMMUNOLOGY, 2003, 170 (04) : 1635 - 1639
  • [4] Lymphocytes in the human gastric mucosa during Helicobacter pylori have a T helper cell 1 phenotype
    Bamford, KB
    Fan, XJ
    Crowe, SE
    Leary, JF
    Gourley, WK
    Luthra, GK
    Brooks, EG
    Graham, DY
    Reyes, VE
    Ernst, PB
    [J]. GASTROENTEROLOGY, 1998, 114 (03) : 482 - 492
  • [5] Immunobiology of dendritic cells
    Banchereau, J
    Briere, F
    Caux, C
    Davoust, J
    Lebecque, S
    Liu, YT
    Pulendran, B
    Palucka, K
    [J]. ANNUAL REVIEW OF IMMUNOLOGY, 2000, 18 : 767 - +
  • [6] Dendritic cells and the control of immunity
    Banchereau, J
    Steinman, RM
    [J]. NATURE, 1998, 392 (6673) : 245 - 252
  • [7] IL-10 and TGF-β induce alloreactive CD4+CD25- T cells to acquire regulatory cell function
    Chen, ZM
    O'Shaughnessy, MJ
    Gramaglia, I
    Panoskaltsis-Mortari, A
    Murphy, WJ
    Narula, S
    Roncarolo, MG
    Blazar, BR
    [J]. BLOOD, 2003, 101 (12) : 5076 - 5083
  • [8] Helicobacter pylori virulence and genetic geography
    Covacci, A
    Telford, JL
    Del Giudice, G
    Parsonnet, J
    Rappuoli, R
    [J]. SCIENCE, 1999, 284 (5418) : 1328 - 1333
  • [9] The role of Helicobacter pylori virulence factors in interleukin production by monocytic cells
    de Jonge, R
    Kusters, JG
    Timmer, MS
    Gimmel, V
    Appelmelk, BJ
    Bereswill, S
    van Vliet, AHM
    Meuwissen, SGM
    Kist, M
    Vandenbroucke-Grauls, CMJE
    Kuipers, EJ
    [J]. FEMS MICROBIOLOGY LETTERS, 2001, 196 (02) : 235 - 238
  • [10] Dendritic cell activation and cytokine production induced by group B Neisseria meningitidis:: Interleukin-12 production depends on lipopolysaccharide expression in intact bacteria
    Dixon, GLJ
    Newton, PJ
    Chain, BM
    Katz, D
    Andersen, SR
    Wong, S
    van der Ley, P
    Klein, N
    Callard, RE
    [J]. INFECTION AND IMMUNITY, 2001, 69 (07) : 4351 - 4357