Quantitative PCR method for sensitive detection of ruminant fecal pollution in freshwater and evaluation of this method in alpine karstic regions

被引：149
作者：
Reischer, Georg H.
Kasper, David C.
Steinborn, Ralf
Mach, Robert L.
Farnleitner, Andreas H.
机构：
[1] Vienna Univ Technol, Inst Chem Engn, Gene Technol Grp, A-1060 Vienna, Austria
[2] Univ vet Med, Inst Anim Breeding & Genet, Dept Anim Breeding & Reprod, A-1210 Vienna, Austria
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2006年 / 72卷 / 08期
基金：
奥地利科学基金会;
关键词：
D O I：
10.1128/AEM.00364-06
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A quantitative TaqMan minor-groove binder real-time PCR assay was developed for the sensitive detection of a ruminant-specific genetic marker in fecal members of the phylum Bacteroidetes. The qualitative and quantitative detection limits determined were 6 and 20 marker copies per PCR, respectively. Tested ruminant feces contained an average of 4.1 x 10(9) marker equivalents per g, allowing the detection of 1.7 ng of feces per filter in fecal suspensions. The marker was detected in water samples from a karstic catchment area at levels matching a gradient from negligible to considerable ruminant fecal influence (from not detectable to 10(5) marker equivalents per liter).
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收藏
页码：5610 / 5614
页数：5
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