Purification, characterization and immunomodulating activity of a polysaccharide from flowers of Abelmoschus esculentus

被引：80
作者：
Zheng, Wei [1 ]
Zhao, Ting [2 ]
Feng, Weiwei [3 ]
Wang, Wei [3 ]
Zou, Ye [3 ]
Zheng, Daheng [3 ]
Takase, Mohammed [2 ]
Li, Qian [1 ]
Wu, Huiyu [1 ]
Yang, Liuqing [2 ]
Wu, Xiangyang [4 ]
机构：
[1] Jiangsu Univ, Sch Pharm, Zhenjiang 212013, Jiangsu, Peoples R China
[2] Jiangsu Univ, Sch Chem & Chem Engn, Zhenjiang 212013, Peoples R China
[3] Jiangsu Univ, Sch Food & Biol Engn, Zhenjiang 212013, Peoples R China
[4] Jiangsu Univ, Sch Environm, Zhenjiang 212013, Jiangsu, Peoples R China
来源：
CARBOHYDRATE POLYMERS | 2014年 / 106卷
关键词：
Okra flowers; Polysaccharide; Immunomodulating activity; Structural characterization; Macrophage activation; SOLUBLE POLYSACCHARIDES; ANTIOXIDANT ACTIVITIES; ANTITUMOR-ACTIVITY; ACTIVATION; MACROPHAGE; EXTRACTION; FRACTIONS; WATER; ATNICOMPLEMENTARY; EXPRESSION;
D O I：
10.1016/j.carbpol.2014.02.079
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
A water-soluble polysaccharide (OFPS11) was obtained from okra (Abelmoschus esculentus) flowers using aqueous extraction and purification with DEAE-52 cellulose and Sephacryl (TM) S-500 column. Its preliminary characterization and immunomodulating activity were investigated. Results showed that OFPS11 is mainly composed of galactose and rhamnose in a molar ratio of 2.23:1 with molecular mass of 1700 kDa. RAW264.7 cells pretreated with OFFS11 significantly inhibited the proliferation of HepG-2 cells. Additionally, OFPS11 enhanced the phagocytic ability and induced the elevation of NO production, TNF-alpha and IL-1 beta secretion of RAW264.7 cells. Furthermore, OFPS11 promoted both the expression of iNOS protein and of iNOS and TNF-alpha mRNA. OFPS11 can strongly increase NF-kappa B levels in nucleuses, which is an important transcription factor that can modulate expressions of iNOS, NO and TNF-alpha. These outcomes support that OFPS11 exerts its antitumor activity by probably stimulating macrophage activities through nuclear NF-kappa B pathway. (C) 2014 Elsevier Ltd. All rights reserved.
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收藏
页码：335 / 342
页数：8
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