Simultaneous determination of glutamate and aspartate in rat periaqueductal gray matter microdialysates by capillary electrophoresis with laser-induced fluorescence

被引：11

作者：

Chen, Hong-Li ^{[1
,2
]}

Zhang, Xiao-Jun ^{[1
]}

Qi, Sheng-Da ^{[1
,2
]}

Xu, Hong-Xi ^{[3
]}

Sung, Joseph J. Y. ^{[4
]}

Bian, Zhao-Xiang ^{[1
]}

机构：

[1] Hong Kong Baptist Univ, Sch Chinese Med, Kowloon Tong, Hong Kong, Peoples R China

[2] Lanzhou Univ, Dept Chem, Lanzhou 730000, Peoples R China

[3] Hong Kong Jockey Club Inst Chinese Med, Hong Kong, Hong Kong, Peoples R China

[4] Chinese Univ Hong Kong, Fac Med, Hong Kong, Hong Kong, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2009年 / 877卷 / 27期

关键词：

Microdialysis; Capillary electrophoresis; Laser-induced fluorescence; 5-Carboxyfluorescein N-succinimidyl ester; Glutamate; Aspartate; AMINO-ACID NEUROTRANSMITTERS; NOXIOUS-STIMULATION; ONLINE; DERIVATIZATION; HYPERALGESIA; RELEASE;

D O I：

10.1016/j.jchromb.2009.08.006

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This study presented a capillary zone electrophoresis (CZE) method for the analysis of trace amount of neurotransmitters glutamate (Glu) and aspartate (Asp) in the microdialysates of rat periaqueductal gray matter (PAG). Glu and Asp were derivatized with the fluorescent agent 5-carboxyfluorescein N-succinimidyl ester (CFSE) for the first time and detected by laser-induced fluorescence (LIF) after separation by CZE. The concentration detection limits (S/N=2) were 6.9 x 10(-10) M and 8.1 x 10(-10) M for Glu and Asp, respectively. The repeatability (expressed as RSD) of the migration times of CFSE-amino acid were better than 0.5%, and not higher than 1.5% even over the period of 1 month. This method was applied to quantify Glu and Asp in rat PAG microdialysates with the treatment of formalin injection, and the measured basal concentrations of Glu and Asp were (22.4 +/- 1.6) x 10(-6) M and (0.9 +/- 0.1) x 10(-6) M, respectively. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：3248 / 3252

页数：5

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