Purification and characterization of a moderately thermostable xylanase from Bacillus sp strain SPS-0

被引：79

作者：

Bataillon, M ^{[1
]}

Cardinali, APN ^{[1
]}

Castillon, N ^{[1
]}

Duchiron, F ^{[1
]}

机构：

[1] Univ Reims Champagne Ardenne, Lab Microbiol Ind, F-51687 Reims 2, France

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 2000年 / 26卷 / 2-4期

关键词：

xylanase; Bacillus; thermophile; purification; characterization; wheat bran;

D O I：

10.1016/S0141-0229(99)00143-X

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A Bacillus spp. strain SPS-0, isolated from a hot spring in Portugal, produced an extracellular xylanase upon growth on wheat bran arabinoxylan. The enzyme was purified to homogeneity by ammonium sulfate precipitation, anion exchange, gel filtration, and affinity chromatography. The optimum temperature and pH for activity was 75 degrees C and 6.0. Xylanase was stable up to 70 degrees C for 4 h at pH 6.0 in the presence of xylane. Xylanase was completely inhibited by the Hg2+ ions. beta-Mercaptoethanol, dithiothreitol, and Mn2+ stimulated the xylanase activity. The products of birchwood xylan hydrolysis were xylose, xylobiose, xylotriose, and xylotetraose. Kinetic experiments at 60 degrees C and pH 6.0 gave V-max and K(m)values of 2420 nkat/mg and 0.7 mg/ml. (C) 2000 Elsevier Science Inc. All rights reserved.

引用

页码：187 / 192

页数：6

共 14 条

[1] INTERLABORATORY TESTING OF METHODS FOR ASSAY OF XYLANASE ACTIVITY [J].

BAILEY, MJ ;

BIELY, P ;

POUTANEN, K .

JOURNAL OF BIOTECHNOLOGY, 1992, 23 (03) :257-270

[2] Microbial xylanolytic enzyme system: Properties and applications [J].

Bajpai, P .

ADVANCES IN APPLIED MICROBIOLOGY, VOL 43, 1997, 43 :141-194

[3]

BARTOLOME B, 1995, BIOTECHNOL APPL BIOC, V22, P65

[4] Extraction and purification of arabinoxylan from destarched wheat bran in a pilot scale [J].

Bataillon, M ;

Mathaly, P ;

Cardinali, APN ;

Duchiron, F .

INDUSTRIAL CROPS AND PRODUCTS, 1998, 8 (01) :37-43

[5] Production of xylanases from a newly isolated alkalophilic thermophilic Bacillus sp. [J].

Bataillon, M ;

Cardinali, APN ;

Duchiron, F .

BIOTECHNOLOGY LETTERS, 1998, 20 (11) :1067-1071

[6]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[7] Purification and characterization of a thermostable xylanase from Bacillus amyloliquefaciens [J].

Breccia, JD ;

Sineriz, F ;

Baigori, MD ;

Castro, GR ;

HattiKaul, R .

ENZYME AND MICROBIAL TECHNOLOGY, 1998, 22 (01) :42-49

[8] PURIFICATION AND CHARACTERIZATION OF A THERMOSTABLE XYLANASE FROM BACILLUS-STEAROTHERMOPHILUS T-6 [J].

KHASIN, A ;

ALCHANATI, I ;

SHOHAM, Y .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1993, 59 (06) :1725-1730

[9] Xylanase production by a new alkali-tolerant isolate of Bacillus [J].

López, C ;

Blanco, A ;

Pastor, FIJ .

BIOTECHNOLOGY LETTERS, 1998, 20 (03) :243-246

[10]

LUEMMLI UK, 1970, NATURE, V227, P680

← 1 2 →