Scavenging Systems for Reactive Carbonyls in the Cyanobacterium Synechocystis sp PCC 6803

被引：18
|
作者：
Shimakawa, Ginga [1 ]
Suzuki, Mayumi [1 ]
Yamamoto, Eriko [1 ]
Nishi, Akiko [1 ]
Saito, Ryota [1 ]
Sakamoto, Katsuhiko [1 ]
Yamamoto, Hiroshi [2 ]
Makino, Amane [3 ,4 ]
Miryake, Chikahiro [1 ,4 ]
机构：
[1] Kobe Univ, Grad Sch Agr Sci, Dept Biol & Environm Sci, Fac Agr,Nada Ku, Kobe, Hyogo 6578501, Japan
[2] Kyoto Univ, Grad Sch Sci, Dept Bot, Sakyo Ku, Kyoto 6068502, Japan
[3] Tohoku Univ, Grad Sch Agr Sci, Dept Agr, Aoba Ku, Sendai, Miyagi 9818555, Japan
[4] Japan Sci & Technol Agcy, Core Res Evolut Sci & Technol, Chiyoda Ku, Tokyo 1020076, Japan
来源：
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2013年 / 77卷 / 12期
基金：
日本学术振兴会;
关键词：
aldo-keto reductase (AKR); cyanobacteria; medium-chain dehydrogenase/reductase (MDR); short-chain dehydrogenase/reductase (SDR); glyoxalase (GLX); ALDO-KETO REDUCTASES; SHORT-CHAIN DEHYDROGENASES/REDUCTASES; CINNAMYL ALCOHOL-DEHYDROGENASE; HUMAN SERUM-ALBUMIN; GLYOXALASE-II; DIABETIC COMPLICATIONS; ARABIDOPSIS-THALIANA; ACTIVE-SITE; PROTEIN CARBONYLATION; SUBSTRATE-SPECIFICITY;
D O I：
10.1271/bbb.130554
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
To elucidate the scavenging systems of sugar- and lipid-derived reactive carbonyls (RCs) in the cyanobacterium Synechocystis sp. PCC 6803 (S. 6803), we selected proteins from S. 6803 based on amino-acid (AA) sequence similarities with proteins from Arabidopsis thaliana, and characterized the properties of the GST-fusion proteins expressed. Slr0942 catalyzed the aldo-keto reductase (AKR) reaction scavenging mainly sugar-derived RCs, methylglyoxal (MG). Slr1192 is the medium-chain dehydrogenase/redutase (MDR). It catalyzed the AKR reaction scavenging several lipid-derived RCs, acrolein, propionaldehyde, and crotonaldehyde. Slr0315 is a short-chain dehydrogenase/redutase (SDR), and it catalyzed only the reduction of MG in the AKR reaction. Slr0381 catalyzed the conversion of hemithioacetal to S-lactoylglutahione (SLG) in the glyoxalase (GLX) 1 reaction. Sll1019 catalyzed the conversion of SLG to glutathione and lactate in the GLX2 reaction. GLX1 and GLX2 compose the glyoxalase system, which scavenges MG. These enzymes contribute to scavenging sugar- and lipid-derived RCs as scavenging systems.
引用
收藏
页码：2441 / 2448
页数：8
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