Site-Directed Mutagenesis Studies on the Toluene Dioxygenase Enzymatic System: Role of Phenylalanine 366, Threonine 365 and Isoleucine 324 in the Chemo-, Regio-, and Stereoselectivity

被引：18

作者：

Agustina Vila, Maria ^{[1
]}

Umpierrez, Diego ^{[1
]}

Veiga, Nicolas ^{[2
]}

Seoane, Gustavo ^{[1
]}

Carrera, Ignacio ^{[1
]}

Rodriguez Giordano, Sonia ^{[1
]}

机构：

[1] Univ Republica, Fac Quim, Lab Biocatalisis & Biotransformac, Dept Biociencias,Dept Quim Organ, Montevideo, Uruguay

[2] Univ Republica, Fac Quim, Dept Estrella Campos, Quim Inorgn, Montevideo, Uruguay

来源：

ADVANCED SYNTHESIS & CATALYSIS | 2017年 / 359卷 / 12期

关键词：

Toluene dioxygenase; cis-cyclohexadienediols; Rieske dioxygenases; site-directed mutagenesis; CIS-DIHYDRODIOL METABOLITES; BIPHENYL DIOXYGENASE; NAPHTHALENE DIOXYGENASE; PSEUDOMONAS-PUTIDA; SUBSTRATE-SPECIFICITY; CATALYZED SYNTHESIS; BINDING-AFFINITY; FLEXIBLE DOCKING; IRON; REGIOSPECIFICITY;

D O I：

10.1002/adsc.201700444

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Toluene Dioxygenase (TDO) enzymatic complex has been widely used as a biocatalyst for the regio- and enantioselective preparation of cis-cyclohexadienediols, which are very important starting materials for organic synthesis. However, the lack of regio- and stereodiversity of the dioxygenation process by TDO and related dioxygenases constitutes a clear drawback when planning the use of these diols in synthetic schemes. In this work, we developed three TDO mutants in residues phenylalanine 366, threonine 365 and isoleucine 324, with the aim to alter the chemo-, regio- and stereoselectivity of the biotransformation of arenes. While no changes in the regioselectivity of the process were observed, dramatic variations in the chemo- and enantioselectivity were found for mutants I324F, T365N and F366 V in a substratedependent manner.

引用

页码：2149 / 2157

页数：9

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