Split Inteins as Versatile Tools for Protein Semisynthesis

被引：101
作者：
Mootz, Henning D. [1 ]
机构：
[1] Tech Univ Dortmund, Fak Chem, D-44227 Dortmund, Germany
来源：
CHEMBIOCHEM | 2009年 / 10卷 / 16期
关键词：
bioconjugation; native chemical ligation; peptides; protein engineering; protein splicing; NATIVE CHEMICAL LIGATION; CELL-SURFACE PROTEINS; IN-VIVO; CRYSTAL-STRUCTURE; DNAE INTEIN; HOMING ENDONUCLEASE; RECOMBINANT PROTEINS; SYNTHETIC PEPTIDE; MINI-INTEIN; RECA INTEIN;
D O I：
10.1002/cbic.200900370
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
(Figure Presented) Divide and conquer: Split inteins link their fused polypeptide sequences with a native peptide bond. This protein trans-splicing (PTS) reaction can be exploited for manifold applications in protein chemistry and biotechnology. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.
引用
收藏
页码：2579 / 2589
页数：11
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