Dynamic organization of DNA replication in one-cell mouse embryos: Relationship to transcriptional activation

被引：67

作者：

BouniolBaly, C ^{[1
]}

Nguyen, E ^{[1
]}

Besombes, D ^{[1
]}

Debey, P ^{[1
]}

机构：

[1] NATL MUSEUM NAT HIST, F-75005 PARIS, FRANCE

来源：

EXPERIMENTAL CELL RESEARCH | 1997年 / 236卷 / 01期

关键词：

D O I：

10.1006/excr.1997.3708

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

We have analyzed the spatial and temporal relationship between transcription and replication sites during the first cell cycle in mouse embryos. Embryos were microinjected with both 5-bromouridine-5'-triphosphate and digoxygenin-11-deoxyuridine-5'-triphosphate to visualize transcription and replication sites respectively. We detected six different phases of replication during S phase and dated the onset of zygotic transcription at the end of the S phase. Using confocal microscopy, we showed that there is essentially no colocalization of replication and transcription sites at this stage of development. Moreover, studies on aphidicolin-treated embryos demonstrated that inhibition of DNA replication does not hinder transcriptional activation at the 1-cell stage. (C) 1997 Academic Press.

引用

页码：201 / 211

页数：11

共 46 条

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