Development of TaqMan real-time polymerase chain reaction for the detection and identification of Penicillium marneffei

被引：19

作者：

Pornprasert, Sakorn ^{[1
]}

Praparattanapan, Jutarat ^{[2
]}

Khamwan, Chantana ^{[3
]}

Pawichai, Sudjai ^{[1
]}

Pimsarn, Parichat ^{[2
]}

Samleerat, Tanawan ^{[1
]}

Leechanachai, Pranee ^{[1
]}

Supparatpinyo, Khunchai ^{[2
]}

机构：

[1] Chiang Mai Univ, Dept Med Technol, Fac Associated Med Sci, Chiang Mai 50200, Thailand

[2] Chiang Mai Univ, Div Infect Dis, Fac Med, Chiang Mai 50200, Thailand

[3] Chiang Mai Univ, Cent Lab Unit, Fac Med, Chiang Mai 50200, Thailand

来源：

MYCOSES | 2009年 / 52卷 / 06期

关键词：

Detection; identification; Penicillium marneffei; real-time PCR; 5; 8S rRNA; TaqMan; SYSTEMIC FUNGAL-INFECTIONS; RAPID IDENTIFICATION; BLOOD CULTURES; PCR ASSAY; DNA; YEAST; DIAGNOSIS; SAMPLES;

D O I：

10.1111/j.1439-0507.2008.01653.x

中图分类号：

R75 [皮肤病学与性病学];

学科分类号：

100206 ;

摘要：

P>Penicillium marneffei is a dimorphic fungus, which is endemic in Southeast Asia and responsible for emerging opportunistic infections. Diagnosis of penicilliosis may be difficult when few yeast cells are present, while a gold standard diagnosis technique requires long-term culture. In order to provide a more rapid and accurate diagnosis, we developed a TaqMan real-time PCR to detect and identify P. marneffei DNA coding for 5.8S rRNA in purified yeast DNA and clinical samples. All P. marneffei DNA preparations could be detected using specific primers and TaqMan probe. The assay has a sensitivity to detect at least 10 yeast cells in seeded blood. Moreover, it can detect P. marneffei DNA in peripheral blood samples and blood-culture bottles. Therefore, the real-time PCR assay may represent a potential tool for early diagnosis of penicilliosis marneffei.

引用

页码：487 / 492

页数：6

共 23 条

[1] Purification and characterization of PCR-inhibitory components in blood cells [J].