Site-specific DNA cleavage on a solid support: A method for mismatch detection

被引：3
|
作者：
Thoeni, Sandra [1 ]
Kressierer, Christoph J. [1 ]
Giese, Bernd [1 ]
机构：
[1] Univ Basel, Dept Chem, CH-4056 Basel, Switzerland
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2007年 / 46卷 / 12期
关键词：
analytical methods; base-pair mismatch; DNA; single nucleotide polymorphisms; solid-phase methods;
D O I：
10.1002/anie.200603092
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
(Figure Presented) Game, set, and mismatch: In a new method for the detection of base-pair mismatches, an immobilized DNA strand with a cleavage site (black square) and a detection tag (red circle) is hybridized with the target strand. If the target nucleotide has a mismatch, then the marker is released from the solid phase (see scheme). © 2007 Wiley-VCH Verlag GmbH & Co. KGaA.
引用
收藏
页码：2112 / 2114
页数：3
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