A Label-free Electrochemical Immunosensor for Highly Sensitive Detection of TNF α, Based on Star Polymer-modified disposable ITO Electrode

被引:2
|
作者
Aydin, Elif Burcu [1 ]
Aydin, Muhammet [1 ]
Sezginturk, Mustafa Kemal [2 ]
机构
[1] Namik Kemal Univ, Sci & Technol Res Ctr, Tekirdag, Turkey
[2] Canakkale Onsekiz Mart Univ, Fac Engn, Bioengn Dept, Canakkale, Turkey
关键词
Tumor necrosis factor alpha; electrochemical immunosensor; single frequencies impedance; star poly(glycidyl methacrylate) polymer; NECROSIS-FACTOR-ALPHA; BIOSENSOR PLATFORM; HUMAN SALIVA; HUMAN SERUM; INTERLEUKIN-1-BETA;
D O I
10.2174/1573412916999200409111759
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Background: Biomarkers are very important disease-related biomolecules which should be analyzed sensitive and selective in related physiological fluids or tissues. Tumor necrosis factor-alpha is a type of cytokine which plays vitlly important roles in different methabolic pathways such as cell death, survival, differentiation, proliferation and migration, and infectious and inflammatory diseases including rheumatoid arthritis, diabetes. Objective: In this study, it was aimed to develop a reliable tool based on star-shaped poly(glycidyl methacrylate) polymer coated disposable indium tin oxide electrode for determination of Tumor necrosis factor-alpha, an important disease biomarker. Methods: Star shaped polymer was used as an interface material for anti- Tumor necrosis factor alpha antibodies immobilization. The antibodies were immobilized covalently onto polymer coated indium tin oxide electrode. Electrochemical impedance spectroscopy and cyclic voltammetry techniques were used for all electrochemical measurements. Results: The suggested immunosensor exhibited a linear range between 0.02 and 4 pg/mL Tumor necrosis factor-alpha, and the detection limit was found as 6 fg/mL. Scanning electron microscopy and atomic force microscopy were used for electrode surface characterization. In addition, the suggested immunosensor was used for Tumor necrosis factor-alpha sensing in human serum samples. The results displayed recoveries between 97.07 and 100.19%. Moreover, this immunosensor had a simple fabrication procedure and a long storage-stability. Conclusion: A new biosensor based on a Star shaped polymer for the ultra sensitive determination of a biomarker Tumor necrosis factor-alpha was developed. The biosensor presented excellent repeatability and reproducubility, and also wide calibration range for Tumor necrosis factor-alpha.
引用
收藏
页码:450 / 459
页数:10
相关论文
共 50 条
  • [11] An Electrochemical Immunosensor Based on a Self-Assembled Monolayer Modified Electrode for Label-Free Detection of α-Synuclein
    Ge, Chuang-Ye
    Rahman, Md. Mahbubur
    Zhang, Wei
    Lopa, Nasrin Siraj
    Jin, Lei
    Yoon, Sujin
    Jang, Hohyoun
    Xu, Guang-Ri
    Kim, Whangi
    SENSORS, 2020, 20 (03)
  • [12] Early detection of the ovarian cancer with a label-free and disposable ITO-PET based immunosensor platform
    Ozcan, Burcu
    Ozay, Hava
    Ozay, O. zguer
    Sezginturk, Mustafa Kemal
    MICROCHEMICAL JOURNAL, 2024, 205
  • [13] Disposable Polyurethane Nanospiked Gold Electrode-Based Label-Free Electrochemical Immunosensor for Clostridium difficile
    Cui, Feiyun
    Zhou, Zhiru
    Feng, Hanping
    Zhou, H. Susan
    ACS APPLIED NANO MATERIALS, 2020, 3 (01) : 357 - 363
  • [14] Label-free electrochemical immunosensor for highly sensitive COVID-19 spike protein detection
    Zhai, Qingfeng
    Wang, Xichu
    Hu, Chuangang
    Zhu, Lin
    Zhang, Chenhao
    Dai, Liming
    SENSORS AND ACTUATORS REPORTS, 2022, 4
  • [15] A novel label-free microfluidic paper-based immunosensor for highly sensitive electrochemical detection of carcinoembryonic antigen
    Wang, Yang
    Xu, Huiren
    Luo, Jinping
    Liu, Juntao
    Wang, Li
    Fan, Yan
    Yan, Shi
    Yang, Yue
    Cai, Xinxia
    BIOSENSORS & BIOELECTRONICS, 2016, 83 : 319 - 326
  • [16] A novel label-free electrochemical immunosensor based on Ce-MOF@AgAuNPs for highly sensitive detection of monensin
    Zhou, Yiting
    Zhou, Jingming
    Liu, Yankai
    Chen, Yumei
    Liu, Hongliang
    Zhu, Xifang
    Ding, Peiyang
    Liang, Chao
    Liu, Enping
    Wu, Sixuan
    Wang, Aiping
    FOOD CONTROL, 2025, 168
  • [17] Label-free electrochemical immunosensor for sensitive detection of pesticide residue in vegetables
    Han, En
    Zhou, Li-Na
    Yan, Jing-Kun
    Cai, Jian-Rong
    Tian, Qing-Rong
    Zeng, Ni
    Modern Food Science and Technology, 2014, 30 (10) : 268 - 273
  • [18] Label-Free Electrochemical Immunosensor Based on Conjugated Polymer Film Coated Disposable Electrode for Ultrasensitive Determination of Resistin Potential Obesity Biomarker
    Aydin, Elif Burcu
    Aydin, Muhammet
    Sezginturk, Mustafa Kemal
    ACS APPLIED BIO MATERIALS, 2024, 7 (03) : 1820 - 1830
  • [19] Label-free electrochemical immunosensor for direct detection of Citrus Tristeza virus using modified gold electrode
    Haji-Hashemi, Hedieh
    Norouzi, Parviz
    Safarnejad, Mohammad Reza
    Ganjali, Mohammad Reza
    SENSORS AND ACTUATORS B-CHEMICAL, 2017, 244 : 211 - 216
  • [20] Rapid detection of Salmonella in milk by a label-free electrochemical immunosensor based on CoFe-MOFs@MWCNTs modified electrode
    Li, Huo
    Huang, Side
    Ling, Tiandi
    Li, Shuang
    Zhang, Yu
    Ying, Yue
    Huang, Ganhui
    Zhang, Jinsheng
    INTERNATIONAL DAIRY JOURNAL, 2025, 166