Characterization of six Merkel cell polyomavirus-positive Merkel cell carcinoma cell lines: Integration pattern suggest that large T antigen truncating events occur before or during integration

被引:44
作者
Schrama, David [1 ]
Sarosi, Eva-Maria [1 ]
Adam, Christian [1 ]
Ritter, Cathrin [2 ,3 ]
Kaemmerer, Ulrike [4 ]
Klopocki, Eva [5 ]
Koenig, Eva-Maria [5 ]
Utikal, Jochen [6 ,7 ]
Becker, Juergen C. [2 ,3 ]
Houben, Roland [1 ]
机构
[1] Univ Hosp Wurzburg, Dept Dermatol, Wurzburg, Germany
[2] Univ Hosp Essen, Dept Translat Skin Canc Res Tscr, Essen, Germany
[3] German Canc Consortium DKTK DKFZ, Heidelberg, Germany
[4] Univ Hosp Wurzburg, Dept Gynecol, Wurzburg, Germany
[5] Univ Wurzburg, Inst Human Genet, Wurzburg, Germany
[6] German Canc Res Ctr, Skin Canc Unit, Heidelberg, Germany
[7] Ruprecht Karl Univ Heidelberg, Univ Med Ctr Mannheim, Dept Dermatol Venereol & Allergol, Mannheim, Germany
关键词
Merkel cell polyomavirus; Merkel cell cancer; integration; concatemer; ASSOCIATION; MUTATIONS; GENOME; CANCER; CGH;
D O I
10.1002/ijc.32280
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Merkel cell carcinoma (MCC), an aggressive neuroendocrine skin tumor, is a polyomavirus-induced human cancer. To study the causal relationship of MCC carcinogenesis with the integrated Merkel cell polyomavirus (MCPyV) in detail, well-characterized MCC cell lines are needed. Consequently, in the current study, we established and characterized six MCPyV-positive MCC cell lines. Microarray-based comparative genomic hybridization revealed a stable genome carrying only a limited number of chromosomal gains and deletions. All cell lines expressed MCC markers Keratin-20 and neuron-specific enolase as well as truncated MCPyV-encoded large T antigen (LT). For five cell lines, we were able to identify the MCPyV-integration sites in introns of different genes. The LT-truncating stop codon mutations and integration sites were affirmed in the respective clinical patient samples. Inverse PCR suggested that three of the cell lines contained MCPyV genomes as concatemers. This notion was confirmed for the two cell lines with known integration sites. Importantly, our observation of distinct stop codon mutations in cell lines with concatemeric MCPyV integration indicates that these LT-truncating mutations occur before integration. In summary, we provide the detailed characterization of six MCPyV-positive MCC cell lines, which are likely to serve as valuable tools in future MCC research.
引用
收藏
页码:1020 / 1032
页数:13
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