Rapid solid-phase peptide synthesis using thermal and controlled microwave irradiation

被引：72
作者：
Bacsa, Bernadett
Desai, Bimbisar
Dibo, Gabor
Kappe, C. Oliver
机构：
[1] Eotvos Lorand Univ, Inst Chem, H-1518 Budapest 112, Hungary
[2] Karl Franzens Univ Graz, Inst Chem, A-8010 Graz, Austria
[3] Karl Franzens Univ Graz, CDLMC, A-8010 Graz, Austria
来源：
JOURNAL OF PEPTIDE SCIENCE | 2006年 / 12卷 / 10期
关键词：
solid-phase peptide synthesis; microwave irradiation; microwave-assisted organic synthesis; microwave-assisted peptide synthesis; MicroKan;
D O I：
10.1002/psc.771
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A rapid and efficient microwave-assisted solid-phase synthesis method is described for the preparation of the nonapeptide WDTVRISFK, using conventional Fmoc/Bu-t orthogonal protection strategy. The synthesis protocol is based on the use of cycles of pulsed microwave irradiation with intermittent cooling of the reaction during the removal of the Fmoc protecting group and during the coupling. The desired nonapeptide was obtained in highest yield and purity by employing MicroKan technology. The chemical reactions were carried out in a single-mode microwave reactor, equipped with a fiber-optic probe to monitor the reaction temperature continuously. Copyright (c) 2006 European Peptide Society and John Wiley & Sons, Ltd.
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页码：633 / 638
页数：6
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