A carbon dot based biosensor for melamine detection by fluorescence resonance energy transfer

被引：160

作者：

Daia, Haichao ^{[1
,2
]}

Shi, Yan ^{[1
,2
]}

Wang, Yilin ^{[1
,2
]}

Sun, Yujing ^{[1
]}

Hu, Jingting ^{[1
,2
]}

Ni, Pengjuan ^{[1
,2
]}

Li, Zhuang ^{[1
]}

机构：

[1] Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Peoples R China

[2] Univ Chinese Acad Sci, Beijing 100039, Peoples R China

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2014年 / 202卷

基金：

中国国家自然科学基金;

关键词：

Melamine; Amino-functionalized carbon dots (C-dots); Fluorescence resonance energy transfer (FRET); Gold nanoparticles (AuNPs); CDTE QUANTUM DOTS; GOLD NANOPARTICLES; RAW-MILK; ALPHA-FETOPROTEIN; TRANSFER FRET; ASSAY; NANODOTS; SURFACE; PHOTOLUMINESCENCE; BIOMOLECULES;

D O I：

10.1016/j.snb.2014.05.058

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In this paper, we constructed a fluorescence resonance energy transfer (FRET) system between amino-functionalized carbon dots (C-dots) and gold nanoparticles (AuNPs). In this system, C-dots were treated as energy donors, while AuNPs were treated as energy acceptors. We optimized some important factors including incubation time, AuNPs concentration and media pH, which would affect the efficiency of the FRET system. Under the optimized experimental conditions, melamine could be detected based on fluorescence intensity of C-dots. We could get a linear relationship between 50 nM and 500 nM and the detection limit was 36 nM. The proposed method was applied to the determination of melamine in milk samples with satisfactory results. Compared with previous reports, the proposed method manifested great advantages including high sensitivity, short analysis time, low cost and ease of operation. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：201 / 208

页数：8

共 37 条

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