Comparative Genomic Profiling of Refractory and Metastatic Penile and Nonpenile Cutaneous Squamous Cell Carcinoma: Implications for Selection of Systemic Therapy

被引：67

作者：

Jacob, Joseph M. ^{[1
]}

Ferry, Elizabeth K.

Gay, Laurie M. ^{[2
]}

Elvin, Julia A. ^{[2
]}

Vergilio, Jo-Anne ^{[2
]}

Ramkissoon, Shakti ^{[2
]}

Severson, Eric ^{[2
]}

Necchi, Andrea ^{[3
]}

Killian, J. Keith ^{[2
]}

Ali, Siraj M. ^{[2
]}

Schrock, Alexa B. ^{[2
]}

Liu, Nick W. ^{[1
]}

Chung, J. ^{[2
]}

Miller, V. A. ^{[2
]}

Stephens, P. J. ^{[2
]}

Welsh, A. ^{[2
]}

Corona, Robert J.

Ross, Jeffrey S. ^{[1
,2
]}

Bratslaysky, Gennady ^{[1
]}

机构：

[1] Upstate Med Univ, Syracuse, NY 13210 USA

[2] Fdn Med, Cambridge, MA USA

[3] Fdn IRCCS Ist Nazl Tumor, Milan, Italy

来源：

JOURNAL OF UROLOGY | 2019年 / 201卷 / 03期

关键词：

penile neoplasms; carcinoma; squamous cell; neoplasm metastasis; genomics; DMA mutational analysis; MUTATIONAL LANDSCAPE; MANAGEMENT; RISK; PROGNOSIS; UPDATE;

D O I：

10.1016/j.juro.2018.09.056

中图分类号：

R5 [内科学]; R69 [泌尿科学（泌尿生殖系疾病）];

学科分类号：

1002 ; 100201 ;

摘要：

Purpose:Metastatic penile squamous cell carcinoma is an aggressive malignancy with limited treatment options. We compared the potential therapy impacting genomic alterations between metastatic penile squamous cell carcinoma and nonpenile metastatic cutaneous squamous cell carcinoma.Materials and Methods:DNA was extracted from 40 mu of formalin fixed, paraffin embedded samples from 78 cases of metastatic penile squamous cell carcinoma and 338 of metastatic cutaneous squamous cell carcinoma. Comprehensive genomic profiling was performed using a hybrid capture, adaptor ligation based, next generation sequencing assay to a mean coverage depth of greater than 500x. The tumor mutational burden was determined on 1.1 Mbp of sequenced DNA and microsatellite instability was determined on 114 loci.Results:Potential targeted therapy opportunities in metastatic penile squamous cell carcinoma cases included alterations in the MTOR pathway (NF1 genomic alterations in 7% and PTEN genomic alterations in 4%) and in the DNA repair pathway (BRCA2 and ATM genomic alterations in 7% each) and tyrosine kinase (EGFR genomic alterations in 6%, and FGFR3 and ERBB2 genomic alterations in 4% each). The tumor mutational burden was significantly higher in predominantly ultraviolet light exposed metastatic squamous cell carcinoma than in metastatic penile squamous cell carcinoma, making metastatic squamous cell carcinoma potentially more responsive to immunotherapies than metastatic penile squamous cell carcinoma. Microsatellite high status was extremely rare for metastatic penile and metastatic cutaneous squamous cell carcinoma. CD274 (PD-L1) amplification was also rare in both tumor types.Conclusions:Metastatic penile squamous cell carcinoma is a unique subtype of squamous cell carcinoma with distinctive genomic features which contrast with those identified in metastatic cutaneous squamous cell carcinoma of nonpenile ultraviolet light exposed skin. Although not rich in predictors of the response to immunotherapy (the tumor mutational burden and microsatellite instability are low), more than a quarter of metastatic penile squamous cell carcinoma cases may potentially benefit from existing and available therapies targeting MTOR, DNA repair and tyrosine kinase pathways.

引用

页码：541 / 548

页数：8

共 28 条

[1] Evaluation of 122 advanced-stage cutaneous squamous cell carcinomas by comprehensive genomic profiling opens the door for new routes to targeted therapies [J].