Human papillomavirus and Epstein-Barr virus infection, p53 expression, and cellular proliferation in laryngeal carcinoma

被引:1
作者
Elgui de Oliveira, Deilson
Bacchi, Maura M.
Macarenco, Ricardo S. S.
Tagliarini, Jose Vicente
Cordeiro, Ricardo C.
Bacchi, Carlos E.
机构
[1] UNESP, Botucatu Sch Med, Dept Pathol, BR-18618917 Botucatu, SP, Brazil
[2] UNESP, Botucatu Sch Med, Dept Otorhinolaryngol Head & Neck Surg, BR-18618917 Botucatu, SP, Brazil
[3] Univ Estadual Campinas, Sch Med Sci, Dept Prevent & Social Med, Campinas, Brazil
关键词
laryngeal cancer; human papillomavirus; HPV; Epstein-Barr virus; EBV; p53; tumor growth fraction;
D O I
10.1039/UU2JADUEHDWATVM9
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
Laryngeal carcinomas are aggressive neoplasms with controversial association with the human papillomavirus (HPV) and Epstein-Barr virus (EBV). So far, the impairment of p53 protein function and its impact on cellular proliferation has not been studied adequately in these tumors. In this work, molecular biologic techniques were used to assess the frequency of HPV and EBV in 110 squamous cell carcinomas of the larynx. In addition, accumulation of p53 and Ki-67 cell proliferation antigen expression in malignant cells was assessed by immunohistochemical analysis. High-grade HPV was found in 37.3% of cases, and none had demonstrable EBV infection. Accumulation of p53 was found in 78.2% of the cases, and it was related to a high Ki-67 labeling index and higher histologic grade. The results demonstrate association of HPV with more than one third of laryngeal carcinomas studied, mainly glottic tumors. Tumors with increased cell proliferation were more frequently high grade, with p53 accumulation and lymph node metastasis.
引用
收藏
页码:284 / 293
页数:10
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