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Enhanced vibrational spectroscopy, intracellular refractive indexing for label-free biosensing and bioimaging by multiband plasmonic-antenna array
被引:22
作者:

Chen, Cheng-Kuang
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Natl Tsing Hua Univ, Dept Mat Sci & Engn, Hsinchu 30013, Taiwan Natl Tsing Hua Univ, Dept Mat Sci & Engn, Hsinchu 30013, Taiwan

Chang, Ming-Hsuan
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Natl Tsing Hua Univ, Dept Mat Sci & Engn, Hsinchu 30013, Taiwan Natl Tsing Hua Univ, Dept Mat Sci & Engn, Hsinchu 30013, Taiwan

Wu, Hsieh-Ting
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Natl Tsing Hua Univ, Dept Mat Sci & Engn, Hsinchu 30013, Taiwan Natl Tsing Hua Univ, Dept Mat Sci & Engn, Hsinchu 30013, Taiwan

Lee, Yao-Chang
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机构:
Natl Synchrotron Radiat Res Ctr, Hsinchu 30076, Taiwan Natl Tsing Hua Univ, Dept Mat Sci & Engn, Hsinchu 30013, Taiwan

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机构:
[1] Natl Tsing Hua Univ, Dept Mat Sci & Engn, Hsinchu 30013, Taiwan
[2] Natl Synchrotron Radiat Res Ctr, Hsinchu 30076, Taiwan
[3] Natl Tsing Hua Univ, Ctr Nanotechnol Mat Sci & Microsysyt, Hsinchu 30013, Taiwan
关键词:
Label-free imaging;
Multiband plasmonic resonances;
Plasmonic antenna;
Refractive index sensing;
Vibrational spectroscopy;
INFRARED-ABSORPTION SPECTROSCOPY;
RESONANCE;
LIGHT;
NANOPARTICLE;
PHONON;
MODES;
D O I:
10.1016/j.bios.2014.04.019
中图分类号:
Q6 [生物物理学];
学科分类号:
071011 ;
摘要:
In this study, we report a multiband plasmonic-antenna array that bridges optical biosensing and intracellular bioimaging without requiring a labeling process or coupler. First, a compact plasmonic-antenna array is designed exhibiting a bandwidth of several octaves for use in both multi-band plasmonic resonance-enhanced vibrational spectroscopy and refractive index probing. Second, a single-element plasmonic antenna can be used as a multifunctional sensing pixel that enables mapping the distribution of targets in thin films and biological specimens by enhancing the signals of vibrational signatures and sensing the refractive index contrast. Finally, using the fabricated plasmonic-antenna array yielded reliable intracellular observation was demonstrated from the vibrational signatures and intracellular refractive index contrast requiring neither labeling nor a coupler. These unique features enable the plasmonic-antenna array to function in a label-free manner, facilitating bio-sensing and imaging development. (C) 2014 Elsevier B.V. All rights reserved.
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页码:343 / 350
页数:8
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