PROTEIN CARBONYLATION AS A MAJOR HALLMARK OF OXIDATIVE DAMAGE: UPDATE OF ANALYTICAL STRATEGIES

被引：393

作者：

Fedorova, Maria ^{[1
,2
]}

Bollineni, Ravi Chand ^{[1
,2
]}

Hoffmann, Ralf ^{[1
,2
]}

机构：

[1] Univ Leipzig, Inst Bioanalyt Chem, Fac Chem & Mineral, D-04103 Leipzig, Germany

[2] Univ Leipzig, Ctr Biotechnol & Biomed BBZ, D-04103 Leipzig, Germany

来源：

MASS SPECTROMETRY REVIEWS | 2014年 / 33卷 / 02期

关键词：

TANDEM MASS-SPECTROMETRY; 2-DIMENSIONAL LIQUID-CHROMATOGRAPHY; HISTIDINE-CONTAINING PEPTIDES; STRESS-INDUCED CARBONYLATION; METAL-CATALYZED OXIDATION; LOSS-DRIVEN MS3; OXIDIZED PROTEINS; REACTIVE OXYGEN; LIPID-PEROXIDATION; PROTEOMIC IDENTIFICATION;

D O I：

10.1002/mas.21381

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

Protein carbonylation, one of the most harmful irreversible oxidative protein modifications, is considered as a major hallmark of oxidative stress-related disorders. Protein carbonyl measurements are often performed to assess the extent of oxidative stress in the context of cellular damage, aging and several age-related disorders. A wide variety of analytical techniques are available to detect and quantify protein-bound carbonyls generated by metal-catalyzed oxidation, lipid peroxidation or glycation/glycoxidation. Here we review current analytical approaches for protein carbonyl detection with a special focus on mass spectrometry-based techniques. The utility of several carbonyl-derivatization reagents, enrichment protocols and especially advanced mass spectrometry techniques are compared and discussed in detail. Furthermore, the mechanisms and biology of protein carbonylation are summarized based on recent high-throughput proteomics data. Copyright © 2013 Wiley Periodicals, Inc.

引用

页码：79 / 97

页数：19

共 179 条

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