Kinetic study and production of N-carbamoyl-D-phenylglycine by immobilized D-hydantoinase from Vigna angularis

被引：15
作者：
Arcuri, MB
Sabino, SJ
Antunes, OAC
Oestreicher, EG
机构：
[1] Univ Bras, Inst Quim, Lab 536B, BR-21945970 Rio De Janeiro, Brazil
[2] Univ Bras, Inst Quim, Lab 641, BR-21945970 Rio De Janeiro, Brazil
来源：
CATALYSIS LETTERS | 2002年 / 79卷 / 1-4期
关键词：
biocatalysis; D-hydantoinase; D-amino acids; Vigna angularis; N-carbamoyl-D-phenylglycine;
D O I：
10.1023/A:1015350106559
中图分类号：
O64 [物理化学（理论化学）、化学物理学];
学科分类号：
070304 ; 081704 ;
摘要：
D-hydantoinase from Vigna angularis was covalently linked to aminopropyl glass beads. Comparative kinetic studies between immobilized and free D-hydantoinase showed that the immobilization procedure did not modify the catalytic properties nor the substrate specificity of the enzyme but increased its stability. In addition, N-carbamoyl-D-phenylglycine was produced in good yield with enantiomeric excess higher than 98%.
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页码：17 / 19
页数：3
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