Single nucleotide polymorphisms of the genes encoding IL-10 and TGF-β1 in Iranian children with atopic dermatitis

被引：12

作者：

Behniafard, N. ^{[1
,2
]}

Amirzargar, A. A. ^{[3
,4
]}

Gharagozlou, M. ^{[1
]}

Delavari, F. ^{[2
,5
]}

Hosseinverdi, S. ^{[2
]}

Sotoudeh, S. ^{[1
]}

Farhadi, E. ^{[6
]}

Mahmoudi, M. ^{[7
,8
]}

Khaledi, M. ^{[9
]}

Moghaddam, Z. G. ^{[1
]}

Aghamohammadi, A. ^{[1
,2
]}

Rezaei, N. ^{[2
,3
,10
]}

机构：

[1] Univ Tehran Med Sci, Pediat Ctr Excellence, Childrens Med Ctr, Tehran, Iran

[2] Univ Tehran Med Sci, Res Ctr Immunodeficiencies, Childrens Med Ctr, Tehran, Iran

[3] Univ Tehran Med Sci, Sch Med, Dept Immunol, Tehran, Iran

[4] Univ Tehran Med Sci, Mol Immunol Res Ctr, Tehran, Iran

[5] USERN, NIIMA, Tehran, Iran

[6] Iran Univ Med Sci, Sch Allied Med Sci, Hematol Dept, Tehran, Iran

[7] Univ Tehran Med Sci, Sch Nutr & Dietet, Tehran, Iran

[8] USERN, Dietitians & Nutr Expert Team DiNET, Tehran, Iran

[9] Univ Tehran Med Sci, Growth & Dev Res Ctr, Tehran, Iran

[10] USERN, NIIMA, Boston, MA 02115 USA

来源：

ALLERGOLOGIA ET IMMUNOPATHOLOGIA | 2018年 / 46卷 / 02期

关键词：

TGF-beta; 1; IL-10; Polymorphisms; Atopic dermatitis; GROWTH-FACTOR-BETA; MESSENGER-RNA; TGF-BETA; INTERLEUKIN-10; CELLS; ASSOCIATION; PROMOTER; INFLAMMATION; EXPRESSION; CYTOKINES;

D O I：

10.1016/j.aller.2017.05.007

中图分类号：

R392 [医学免疫学];

学科分类号：

100102 ;

摘要：

Background: Atopic dermatitis is an inflammatory skin disease in which both genetic and environmental factors interact to determine the susceptibility and severity of the disease. Objective: The aim of this study was to determine the association between atopic dermatitis and IL-10 and TGF-beta 1 gene polymorphisms. Methods: The allele and genotype frequencies of genes encoding for IL-10 and TGF-beta 1 were investigated in 89 patients with atopic dermatitis in comparison with 138 in the control group using the PCR-SSP method. Results: A significant increase was found in the frequency of the TGF-beta 1 codon 10/C allele among patients (p<0.001, OR = 6.77), whereas a significant decrease was observed in the frequency of the T allele at the same position (p < 0.001, OR = 0.14). The frequency of the TGF-beta 1 codon 25/G allele in the control group was significantly higher than among patients (p<0.001, OR = 0.08). A significant positive correlation was seen between CC (p < 0.001, OR = 15.10) and CG (p < 0.001) genotypes and AD at codons 10 and 25, respectively. The most frequent haplotypes among patients was TGF-beta 1 CG which was significantly higher than in the control subjects (50% in patients vs. 39.9% in controls, p = 0.042). A significant increase was found in the frequency of TGF-beta CC (36% in patients vs. 7.6% in controls, p < 0.001) and TC (14% in patients vs. 0% in controls, p < 0.001) haplotypes among patients compared to controls. By contrast, the TGF-beta 1 TG haplotype was significantly lower in patients than controls (0% in patients vs. 52.5% in controls, p < 0.001). There were no significant differences in the frequency of alleles, genotypes and haplotypes of the IL-10 gene. Conclusions We found a strong association between the polymorphisms of the TGF-beta 1 gene at codon 10 and codon 25 positions and atopic dermatitis. (C) 2017 SEICAP. Published by Elsevier Espana, S.L.U. All rights reserved.

引用

页码：155 / 159

页数：5

共 37 条

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