Effects of Maslinic Acid, a Natural Triterpene, on Glycogen Metabolism in Cultured Cortical Astrocytes

被引：11

作者：

Guan, Teng ^{[1
]}

Li, Yunman ^{[1
]}

Sun, Hongbin ^{[2
]}

Tang, Xuzhen ^{[1
]}

Qian, Yisong ^{[1
]}

机构：

[1] China Pharmaceut Univ, Dept Physiol, Nanjing 210009, Peoples R China

[2] China Pharmaceut Univ, Ctr Drug Discovery, Nanjing 210009, Peoples R China

来源：

PLANTA MEDICA | 2009年 / 75卷 / 10期

关键词：

maslinic acid; pentacyclic triterpene; glycogen phosphorylase inhibitor; astrocytes; OCCURRING PENTACYCLIC TRITERPENES; ENERGY-METABOLISM; PHOSPHORYLASE; INHIBITORS;

D O I：

10.1055/s-0029-1185481

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Maslinic acid (2-alpha,3-beta-dihydroxyolean-12-en-28-oic acid) is a triterpenoid compound present in plants of Olea europaea. In the present study, we investigated the effect of maslinic acid on astrocytic glycogen metabolism. Glycogen phosphorylase (GP) activity in homogenates of cultured astrocytes was analyzed, and maslinic acid exhibited GP inhibition with an IC50 value of 5.7 mu M. Moreover, the influence of maslinic acid on glycogen synthesis and glycogenolysis was also investigated. Pre-incubation with maslinic acid dose-dependently increased cellular glycogen content and prevented the excessive glycogenolysis induced by norepinephrine. In conclusion, maslinic acid is suggested to be a potent inhibitor of astrocytic glycogen phosphorylase.

引用

页码：1141 / 1143

页数：3

共 13 条

[1] Hypoglycemic seizures during transient hypoglycemia exacerbate hippocampal dysfunction [J].

Abdelmalik, Peter A. ;

Shannon, Patrick ;

Yiu, Adelaide ;

Liang, Philip ;

Adamchik, Yana ;

Weisspapir, Michael ;

Samoilova, Marina ;

Burnham, W. McIntyre ;

Carlen, Peter L. .

NEUROBIOLOGY OF DISEASE, 2007, 26 (03) :646-660

[2] Inactivation of phosphorylase is a major component of the mechanism by which insulin stimulates hepatic glycogen synthesis [J].

Aiston, S ;

Coghlan, MP ;

Agius, L .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 2003, 270 (13) :2773-2781

[3]

BERGMEYER HU, 1974, METHOD ENZYMAT AN, P293

[4] Astrocytic involvement in learning and memory consolidation [J].

Gibbs, Marie E. ;

Hutchinson, Dana ;

Hertz, Leif .

NEUROSCIENCE AND BIOBEHAVIORAL REVIEWS, 2008, 32 (05) :927-944

[5] BIOCHEMICAL AND ULTRASTRUCTURAL-STUDY OF GLYCOGEN IN CULTURED ASTROCYTES SUBMITTED TO THE CONVULSANT METHIONINE SULFOXIMINE [J].

HEVOR, TK ;

DELORME, P .

GLIA, 1991, 4 (01) :64-69

[6] Energy metabolism and memory processing: Role of glucose transport and glycogen in responses to adrenoceptor activation in the chicken [J].

Hutchinson, Dana S. ;

Summers, Roger J. ;

Gibbs, Marie E. .

BRAIN RESEARCH BULLETIN, 2008, 76 (03) :224-234

[7]

LOWRY OH, 1951, J BIOL CHEM, V193, P265

[8] PREPARATION OF SEPARATE ASTROGLIAL AND OLIGODENDROGLIAL CELL-CULTURES FROM RAT CEREBRAL TISSUE [J].

MCCARTHY, KD ;

DEVELLIS, J .

JOURNAL OF CELL BIOLOGY, 1980, 85 (03) :890-902

[9]

NEESE J, 1974, CLIN CHEM, V20, P878

[10] Glycogen phosphorylase isozymes and energy metabolism in the rat peripheral nervous system - An immunocytochemical study [J].

Pfeiffer-Guglielmi, Brigitte ;

Francke, Mike ;

Reichenbach, Andreas ;

Hamprecht, Bernd .

BRAIN RESEARCH, 2007, 1136 (01) :20-27

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