Approach to ecofriendly leather: characterization and application of an alkaline protease for chemical free dehairing of skins and hides at pilot scale

被引：60

作者：

George, Nancy ^{[1
]}

Chauhan, Prakram Singh ^{[1
]}

Kumar, Vivek ^{[1
]}

Puri, Neena ^{[2
]}

Gupta, Naveen ^{[1
]}

机构：

[1] Panjab Univ, Dept Microbiol, Chandigarh 160014, India

[2] Guru Nanak Khalsa Coll, Dept Ind Microbiol, Yamunanagar, Haryana, India

来源：

JOURNAL OF CLEANER PRODUCTION | 2014年 / 79卷

关键词：

Vibrio metschnikovii; Purification; Dehairing; Leather industry; RECENT TRENDS; LIME;

D O I：

10.1016/j.jclepro.2014.05.046

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The objective of this work was to purify and characterize alkaline protease from Vibrio metschnikovii NG 155 and to study its applicability as a dehairing agent at pilot scale in leather industry. Relative molecular weight of the purified protease was found to be 45 kDa with optimum temperature and pH of 65 degrees C and 10 respectively. Stability of enzyme in wide range of pH (7-11) and temperature (10-50 degrees C) makes it suitable for industrial applications. The protease efficiently removed hair from goat skins and buffalo hides, completely eliminating the use of lime and sulphide within short process duration of 8 h. Efficacy of the developed process is supported by histology, scanning electron microscopic analysis and quantification of different skin components. In addition, better physicochemical properties of dyed crusts and lesser pollution load in dehairing process, further affirms the potential of this enzyme for ecofriendly dehairing of animal skins in leather industry. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：249 / 257

页数：9

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