Target Delivery and Cell Imaging Using Hyaluronic Acid-Functionalized Graphene Quantum Dots

被引:162
作者
Abdullah-Al-Nahain [1 ]
Lee, Jung-Eun [2 ]
In, Insik [3 ]
Lee, Haeshin [4 ]
Lee, Kang Dae [5 ]
Jeong, Ji Hoon [2 ]
Park, Sung Young [6 ]
机构
[1] Korea Natl Univ Transportat, Dept Green Bio Engn, Chungju 380702, South Korea
[2] Sungkyunkwan Univ, Sch Pharm, Suwon 440746, Gyeonggi Do, South Korea
[3] Korea Natl Univ Transportat, Dept Polymer Sci & Engn, Chungju 380702, South Korea
[4] Korea Adv Inst Sci & Technol, Dept Chem, Taejon 305701, South Korea
[5] Kosin Univ, Coll Med, Dept Otolaryngol Head & Neck Surg, Pusan, South Korea
[6] Korea Natl Univ Transportat, Dept Chem & Biol Engn, Chungju 380702, South Korea
关键词
graphene quantum dots; hyaluronic acid; cell imaging; target delivery; in vivo; FLUORESCENCE; CYTOTOXICITY; DOXORUBICIN; OXIDE; DRUG; NANOCRYSTALS; ENHANCEMENT; REDUCTION; ADHESIVE; POLYMER;
D O I
10.1021/mp400219u
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
This work demonstrates the way to achieve efficient and target specific delivery of a graphene quantum dot (GQD) using hyaluronic acid (HA) (GQD-HA) as a targeting agent. HA has been anchored to a GQD that accepts the fascinating adhesive properties of the catechol moiety, dopamine hydrochloride, conjugated to HA, which was confirmed by X-ray photoelectron spectroscopy. Transmission electron microscopy revealed a particle size of similar to 20 nm, and the fluorescence spectra revealed significant fluorescence intensity even after the anchoring of HA. The prepared GQD-HA was applied to CD44 receptor overexpressed tumor-bearing balb/c female mice, and the in vivo biodistribution investigation demonstrated more bright fluorescence from the tumor tissue. In vitro cellular imaging, via a confocal laser scanning microscope, exhibited strong fluorescence from CD44 overexpressed A549 cells. Both in vivo and in vitro results showed the effectiveness of using HA as targeting molecule. The loading and release kinetics of the hydrophobic drug doxorubicin from a GQD under mildly acidic conditions showed that a GQD can be considered as a novel drug carrier, while the nontoxic behavior from the MTT assay strongly supports the identification of GQD-HA as a biocompatible material.
引用
收藏
页码:3736 / 3744
页数:9
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