microRNA-27a functions as a tumor suppressor in esophageal squamous cell carcinoma by targeting KRAS

被引：39

作者：

Zhu, Linan ^{[1
]}

Wang, Zhiju ^{[2
]}

Fan, Qingxia ^{[1
]}

Wang, Ruilin ^{[1
]}

Sun, Yan ^{[1
]}

机构：

[1] Zhengzhou Univ, Dept Oncol, Affiliated Hosp 1, Zhengzhou 450001, Henan, Peoples R China

[2] Zhengzhou Univ, Dept Basic Med, Zhengzhou 450001, Henan, Peoples R China

来源：

ONCOLOGY REPORTS | 2014年 / 31卷 / 01期

关键词：

miR-27a; esophageal squamous cell carcinoma; tumor suppressor; KRAS; EXPRESSION; CANCER; RAS; PROMOTES; GENE; ADENOCARCINOMA; PROLIFERATION; INVASION; MIR-27A; EGFR;

D O I：

10.3892/or.2013.2807

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

microRNAs (miRNAs) have been suggested to play a vital role in regulating tumor progression and invasion. However, the expression of miR-27a in esophageal squamous cell carcinoma (ESCC) and its effect on the tumorigenesis of ESCC are unclear. In the present study, we found that miR-27a was downregulated in esophageal carcinoma cell lines and ESCC specimens with lymph node metastasis. Furthermore, we demonstrated that miR-27a binds to the 3-untranslated region (UTR) of KRAS and inhibits the expression of the KRAS protein. miR-27a levels were inversely correlated with levels of KRAS mRNA and protein in ESCC specimens. Both in vitro and in vivo assays revealed that miR-27a attenuated ESCC proliferation, invasion and tumor growth in nude mice. miR-27a exerts its tumor suppressor function through inhibition of the KRAS-related ERK pathways. Our findings suggest, for the first time, that miR-27a suppresses tumorigenesis of ESCC by targeting KRAS.

引用

收藏

页码：280 / 286

页数：7

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