microRNA-27a functions as a tumor suppressor in esophageal squamous cell carcinoma by targeting KRAS

被引：39
作者：
Zhu, Linan [1 ]
Wang, Zhiju [2 ]
Fan, Qingxia [1 ]
Wang, Ruilin [1 ]
Sun, Yan [1 ]
机构：
[1] Zhengzhou Univ, Dept Oncol, Affiliated Hosp 1, Zhengzhou 450001, Henan, Peoples R China
[2] Zhengzhou Univ, Dept Basic Med, Zhengzhou 450001, Henan, Peoples R China
来源：
ONCOLOGY REPORTS | 2014年 / 31卷 / 01期
关键词：
miR-27a; esophageal squamous cell carcinoma; tumor suppressor; KRAS; EXPRESSION; CANCER; RAS; PROMOTES; GENE; ADENOCARCINOMA; PROLIFERATION; INVASION; MIR-27A; EGFR;
D O I：
10.3892/or.2013.2807
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
microRNAs (miRNAs) have been suggested to play a vital role in regulating tumor progression and invasion. However, the expression of miR-27a in esophageal squamous cell carcinoma (ESCC) and its effect on the tumorigenesis of ESCC are unclear. In the present study, we found that miR-27a was downregulated in esophageal carcinoma cell lines and ESCC specimens with lymph node metastasis. Furthermore, we demonstrated that miR-27a binds to the 3-untranslated region (UTR) of KRAS and inhibits the expression of the KRAS protein. miR-27a levels were inversely correlated with levels of KRAS mRNA and protein in ESCC specimens. Both in vitro and in vivo assays revealed that miR-27a attenuated ESCC proliferation, invasion and tumor growth in nude mice. miR-27a exerts its tumor suppressor function through inhibition of the KRAS-related ERK pathways. Our findings suggest, for the first time, that miR-27a suppresses tumorigenesis of ESCC by targeting KRAS.
引用
收藏
页码：280 / 286
页数：7
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