Properties of the coat protein of the tobacco mosaic virus Kazakh isolate K1

被引：0

作者：

Novikov, VK ^{[1
]}

Dobrov, EN

Belenovich, EV

Zavriev, SK

机构：

[1] Moscow MV Lomonosov State Univ, Belozersky Inst Physicochem Biol, Moscow 119899, Russia

[2] Inst Agr Biotechnol, Moscow 127550, Russia

来源：

MOLECULAR BIOLOGY | 2000年 / 34卷 / 02期

基金：

俄罗斯基础研究基金会;

关键词：

tobacco mosaic virus; coat protein; specific and nonspecific aggregation; reconstruction;

D O I：

10.1007/BF02759653

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A study was made of the coat protein (CP) of thermosensitive semidefective tobacco mosaic virus strain K1 (TMV-K1). In contrast to CP of other TMV strains, K1 CP showed high nonspecific aggregation and did not form normal two-layered cylindrical aggregates. In none of the conditions tested, K1 CP formed virions with cognate K1 RNA in vitro. The abnormal properties were attributed to substitution Lys53 --> Glu differentiating the K1 CP from those of other tobamoviruses. It is assumed that the high structural plasticity allows the tobamovirus virions to incorporate CP subunits even with unfavorable amino acid changes.

引用

页码：286 / 290

页数：5

共 16 条

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