Gene Regulation with Polyvalent siRNA-Nanoparticle Conjugates

被引：522
作者：
Giljohann, David A.
Seferos, Dwight S.
Prigodich, Andrew E.
Patel, Pinal C.
Mirkin, Chad A. [1 ]
机构：
[1] Northwestern Univ, Dept Chem, Evanston, IL 60208 USA
来源：
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2009年 / 131卷 / 06期
关键词：
MODIFIED GOLD NANOPARTICLES; OPTICAL-PROPERTIES; NUCLEIC-ACIDS; DELIVERY; PROBES; CRYSTALLIZATION; ASSEMBLIES; CELLS;
D O I：
10.1021/ja808719p
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
We report the synthesis and characterization of polyvalent RNA-gold nanoparticle conjugates (RNA-Au NPs), nanoparticles that are densely functionalized with synthetic RNA oligonucleotides and designed to function in the RNAi pathway. The particles were rationally designed and synthesized to be free of degrading enzymes, have a high surface loading of siRNA duplexes, and contain an auxiliary passivating agent for increased stability in biological media. The resultant conjugates have a half-life six times longer than that of free dsRNA, readily enter cells without the use of transfection agents, and demonstrate a high gene knockdown capability in a cell model.
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页码：2072 / +
页数：3
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