Facilitated Transport of Ions and Glucose by Amphotericin B Across Lipid Bilayers in the Presence or Absence of Cholesterol

被引：1

作者：

Kagohashi, Hiroki ^{[1
]}

Shirai, Osamu ^{[1
]}

Kubota, Shintaro ^{[1
]}

Kitazumi, Yuki ^{[1
]}

Kano, Kenji ^{[1
]}

机构：

[1] Kyoto Univ, Div Appl Life Sci, Grad Sch Agr, Kyoto 6068502, Japan

来源：

ELECTROANALYSIS | 2014年 / 26卷 / 03期

关键词：

Amphotericin B; Bilayer lipid membrane; Ion transport; Liposome; Glucose; Cholesterol; MEMBRANES; DEPENDENCE; ERGOSTEROL; GRAMICIDIN; LIPOSOMES; NYSTATIN; CHANNEL; STEROL; PORES;

D O I：

10.1002/elan.201300555

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The transport of ions and glucose across bilayer lipid membranes (BLM) facilitated by amphotericin B (AmB) is studied by use of planar BLMs and liposomal membranes. The transport characteristics change with time in the presence of cholesterol, while it is independent of time in the absence of cholesterol. The carrier-type transport is observed immediately after the addition of AmB. In the presence of cholesterol, AmB forms a 1:1 complex with cholesterol and the channel is formed by aggregation of AmB-cholesterol complexes. It is concluded that the number of the channels increases with time and that the carrier-type transport decreases instead.

引用

页码：625 / 631

页数：7

共 21 条

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