The putative stress sensor protein MtlA is required for conidia formation, cell wall stress tolerance, and cell wall integrity in Aspergillus nidulans

被引:16
作者
Futagami, Taiki [1 ]
Seto, Kazufumi [1 ]
Kajiwara, Yasuhiro [2 ]
Takashita, Hideharu [2 ]
Omori, Toshiro [2 ]
Takegawa, Kaoru [1 ]
Goto, Masatoshi [1 ]
机构
[1] Kyushu Univ, Fac Agr, Dept Biosci & Biotechnol, Fukuoka 812, Japan
[2] Sanwa Shurui Co Ltd, Res & Dev Lab, Usa, Japan
基金
日本学术振兴会;
关键词
Aspergillus nidulans; cell wall integrity; stress sensor; MtlA; KINASE-C; SACCHAROMYCES-CEREVISIAE; POLARIZED GROWTH; PKCA; IDENTIFICATION; GLYCOSYLATION; INVOLVEMENT; BIOGENESIS; PATHWAY; GENE;
D O I
10.1080/09168451.2014.878218
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The Mid2-like protein MtlA is a putative sensor of the cell wall integrity (CWI) signaling pathway in Aspergillus nidulans. An MtlA-EGFP fusion protein was localized at the cell surface and septa. The mtlA disruptant (Delta mtlA) showed radial colony growth similar to the wild-type (wt) strain, but showed reduced conidia formation. The Delta mtlA mutant showed growth deficiency in the presence of inhibitors of cell wall synthesis. Moreover, mtlA disruption resulted in a reduction in the glucan and chitin content in the cell wall. These results suggest that MtlA plays a significant role in asexual sporulation, cell wall stress tolerance, and the maintenance of CWI in A. nidulans, but transcriptional upregulation of alpha-1,3-glucan synthase gene agsB induced by micafungin was observed in the Delta mtlA strain as well as the wt strain. Thus, MtlA is not essential for activation of the downstream CWI signaling pathway components identified in previous studies of Saccharomyces cerevisiae.
引用
收藏
页码:326 / 335
页数:10
相关论文
共 33 条
[1]   CHARACTERIZATION OF ASPERGILLUS-NIDULANS MUTANTS DEFICIENT IN CELL-WALL CHITIN OR GLUCAN [J].
BORGIA, PT ;
DODGE, CL .
JOURNAL OF BACTERIOLOGY, 1992, 174 (02) :377-383
[2]   Involvement of the Aspergillus nidulans protein kinase C with farnesol tolerance is related to the unfolded protein response [J].
Colabardini, Ana Cristina ;
de Castro, Patricia Alves ;
de Gouvea, Paula Fagundes ;
Savoldi, Marcela ;
Malavazi, Iran ;
Goldman, Maria Helena S. ;
Goldman, Gustavo Henrique .
MOLECULAR MICROBIOLOGY, 2010, 78 (05) :1259-1279
[3]   Deciphering cell wall integrity signalling in Aspergillus fumigatus: identification and functional characterization of cell wall stress sensors and relevant Rho GTPases [J].
Dichtl, Karl ;
Helmschrott, Christoph ;
Dirr, Franziska ;
Wagener, Johannes .
MOLECULAR MICROBIOLOGY, 2012, 83 (03) :506-519
[4]  
Fontaine T, 2000, J BIOL CHEM, V275, P41528
[5]   MpkA-dependent and -independent cell wall integrity signaling in Aspergillus nidulans [J].
Fujioka, Tomonori ;
Mizutani, Osamu ;
Furukawa, Kentaro ;
Sato, Natsuko ;
Yoshimi, Akira ;
Yamagata, Youhei ;
Nakajima, Tasuku ;
Abe, Keietsu .
EUKARYOTIC CELL, 2007, 6 (08) :1497-1510
[6]  
Futagami Taiki, 2012, Commun Integr Biol, V5, P206, DOI 10.4161/cib.18993
[7]   Putative Stress Sensors WscA and WscB Are Involved in Hypo-Osmotic and Acidic pH Stress Tolerance in Aspergillus nidulans [J].
Futagami, Taiki ;
Nakao, Seiki ;
Kido, Yayoi ;
Oka, Takuji ;
Kajiwara, Yasuhiro ;
Takashita, Hideharu ;
Omori, Toshiro ;
Furukawa, Kensuke ;
Goto, Masatoshi .
EUKARYOTIC CELL, 2011, 10 (11) :1504-1515
[8]   Protein O-glycosylation in fungi:: Diverse structures and multiple functions [J].
Goto, Masatoshi .
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2007, 71 (06) :1415-1427
[9]   Protein O-Mannosyltransferases B and C Support Hyphal Development and Differentiation in Aspergillus nidulans [J].
Goto, Masatoshi ;
Harada, Yuka ;
Oka, Takuji ;
Matsumoto, Sho ;
Takegawa, Kaoru ;
Furukawa, Kensuke .
EUKARYOTIC CELL, 2009, 8 (10) :1465-1474
[10]   Aspergillus nidulans RhoA is involved in polar growth, branching, and cell wall synthesis [J].
Guest, GM ;
Lin, XR ;
Momany, M .
FUNGAL GENETICS AND BIOLOGY, 2004, 41 (01) :13-22