Effects of Physico-Chemical Parameters on Actinomycetes Communities during Composting of Agricultural Waste

被引:3
作者
Li, Yuanping [1 ]
Chen, Yanrong [2 ,3 ]
Chen, Yaoning [2 ,3 ]
Wu, Yanxin [2 ,3 ]
Zhang, Chun [1 ]
Peng, Zhen [2 ,3 ]
Liu, Yihuan [2 ,3 ]
Wang, Sha [2 ,3 ]
Xu, Ran [2 ,3 ]
Zeng, Ziping [2 ,3 ]
机构
[1] Hunan City Univ, Coll Municipal & Mapping Engn, Yiyang 413000, Peoples R China
[2] Hunan Univ, Coll Environm Sci & Engn, Changsha 410082, Hunan, Peoples R China
[3] Hunan Univ, Minist Educ, Key Lab Environm Biol & Pollut Control, Changsha 410082, Hunan, Peoples R China
来源
SUSTAINABILITY | 2019年 / 11卷 / 08期
关键词
composting; Actinomycetes communities; PCR-DGGE; quantitative PCR; regression analysis; redundancy analysis; GRADIENT GEL-ELECTROPHORESIS; MICROBIAL COMMUNITY; PHANEROCHAETE-CHRYSOSPORIUM; MIXED CULTURE; RICE STRAW; DGGE; BIODEGRADATION; DIVERSITY; INOCULATION; DEGRADATION;
D O I
10.3390/su11082229
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
The objective of this study was to investigate the influence of physico-chemical parameters on Actinomycetes communities and to prioritize those parameters that contributed to Actinomycetes community composition during the composting of agricultural waste. Denaturing gradient gel electrophoresis of polymerase chain reaction (PCR-DGGE) and redundancy analysis (RDA) were used to determine the relationships between those parameters and Actinomycetes community composition. Quantitative PCR (qPCR) and regression analysis were used to monitor the 16S rDNA copy numbers of Actinomycetes and to analyse the correlations between physico-chemical parameters and Actinomyces 16S rDNA gene abundance, respectively. The RDA results showed that moisture content, water soluble carbon (WSC) and pH (p < 0.05) made the main contributions to the temporal variations of Actinomycetes community composition. The output of the regression analysis indicated that moisture content (R-2 = 0.407, p < 0.01) showed a negative linear relationship with the Actinomyces 16S rDNA gene abundance.
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页数:13
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