Manipulation of PrPres production in scrapie-infected neuroblastoma cells

被引:16
作者
Bate, C
Langeveld, J
Williams, A
机构
[1] Univ Glasgow, Sch Vet, Dept Vet Pathol, Inst Comparat Med, Glasgow G61 1QH, Lanark, Scotland
[2] Pepscan Syst BV, NL-8219 PH Lelystad, Netherlands
[3] Univ London Royal Vet Coll, Dept Pathol & Infect Dis, N Mymms AL9 7TA, Herts, England
关键词
prion; scrapie-infected neuroblastoma cells; retinoic acid; microglia; ELISA;
D O I
10.1016/j.jneumeth.2004.04.001
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
In the present study the accumulation of protease resistant prion protein (PrPres,,) in scrapie-infected neuroblastoma cells (ScN2a cells) was shown to be dependent on culture conditions. The highest levels of PrPres were found in slow growing cells. Further increases in PrPres accumulation were observed in ScN2a cells treated with retinoic acid, a compound that is associated with neuronal differentiation. The effects of retinoic acid were dose-dependent with a maximal effect at 200 ng/ml. A similar increase in PrPres was observed in another prion-infected cell line, scrapie-mouse brain (SMB) cells, treated with retinoic acid while retinoic acid increased the amount of PrPC in non-infected cells. Other drugs reported to cause neuronal differentiation, such as phorbol esters, did not increase the PrPres content of ScN2a cells. The survival of retinoic acid-treated ScN2a cells co-cultured with microglia was significantly reduced when compared to untreated ScN2a cells and an inverse correlation was demonstrated between the PrPres content of cells and their survival when co-cultured with microglia. The production of interleukin-6 by microglia cultured with retinoic acid-treated ScN2a cells was significantly higher than that of microglia cultured with untreated ScN2a cells. (C) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码:217 / 223
页数:7
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