Lipid A Structure and Immunoinhibitory Effect of the Marine Bacterium Cobetia pacifica KMM 3879T

被引：14

作者：

Di Lorenzo, Flaviana ^{[1
]}

Palmigiano, Angelo ^{[2
]}

Albitar-Nehme, Sami ^{[3
]}

Pallach, Mateusz ^{[1
]}

Kokoulin, Maxim ^{[4
]}

Komandrova, Nadezhda ^{[4
]}

Romanenko, Lyudmila ^{[4
]}

Bernardini, Maria Lina ^{[3
]}

Garozzo, Domenico ^{[2
]}

Molinaro, Antonio ^{[1
]}

Silipo, Alba ^{[1
]}

机构：

[1] Univ Naples Federico II, Dept Chem Sci, Via Cinthia 4, I-80126 Naples, Italy

[2] CNR, IPCB, Via P Gaifami 18, I-95126 Catania, Italy

[3] Sapienza Univ Rome, Dept Biol & Biotechnol Charles Darwin, Piazzale Aldo Moro 5, I-00185 Rome, Italy

[4] Russian Acad Sci, Far East Branch, GB Elyakov Pacific Inst Bioorgan Chem, Svetlanskaya St 50, Vladivostok 690022, Russia

来源：

EUROPEAN JOURNAL OF ORGANIC CHEMISTRY | 2018年 / 2018卷 / 20-21期

基金：

欧盟地平线“2020”; 俄罗斯基础研究基金会;

关键词：

Bacteria; Lipids; Lipopolysaccharides; Immunology; Structure elucidation; ABSOLUTE-CONFIGURATION; RAPID METHOD; LIPOPOLYSACCHARIDES; LPS; RECOGNITION; COMPLEX;

D O I：

10.1002/ejoc.201800279

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

The structural elucidation of lipopolysaccharides (LPSs) from Gram-negative marine bacteria, along with the assessment of their immunological properties, is a fascinating and active research field. Such studies can aid understanding of adaptation phenomena that occur in the marine environment, but they can also open up new perspectives on the design and development of new immunoregulatory drugs. In this paper, we report the structural characterization of the lipid A component of the LPS isolated from the marine bacterium Cobetia pacifica KMM 3879(T), which is characterized by a family of structures differing in their acylation patterns. The structural assignment was achieved through extensive chemical analysis and matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. Moreover, cellular immunology studies on the LPS highlighted its low immunostimulatory impact, as well as a very interesting and promising inhibitory activity of the toxic effects of Escherichia coli LPS.

引用

页码：2707 / 2716

页数：10

共 28 条

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