Synthesis and study on hydrolytic properties of fluorescein esters

被引：20

作者：

Ge, Feng-Yan

Chen, Li-Gong

Zhou, Xiao-Li

Pan, Hui-Ying

Yan, Fan-Yong

Bai, Guo-Yi

Yan, Xi-Long ^{[1
]}

机构：

[1] Tianjin Univ, Sch Pharmaceut & Biotechnol, Tianjin 300072, Peoples R China

[2] Hebei Univ, Coll Chem & Environm Sci, Baoding 071002, Peoples R China

来源：

DYES AND PIGMENTS | 2007年 / 72卷 / 03期

关键词：

fluorescein esters; fluorescein dilaurate; spontaneous hydrolysis; enzymatic hydrolysis;

D O I：

10.1016/j.dyepig.2005.09.012

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A series of fluorescein esters of straight chain fatty acid were synthesized and applied to the fluorometric assay of lipase. The rate of change in the fluorescence of the solution, due to the production of fluorescein, was measured and correlated with enzyme activity. The hydrolytic properties of fluorescein esters were investigated. In addition, the relationship between their hydrolytic properties and the chain length of fluorescein esters was discussed in this paper. In contrast to fluorescein dibutyrate, fluorescein dilaurate was found to be a better substrate for the assay of lipase with the higher rate of hydrolysis and better K-m value. As little as 0.0001 mg/ml of lipase can be detected with a relative accuracy of about 1.5%. (c) 2005 Elsevier Ltd. All rights reserved.

引用

页码：322 / 326

页数：5

共 19 条

[1] Design and synthesis of a fluorescent reporter of protein kinase activity [J].