Microfluidic analysis of circulating tumor cells and tumor-derived extracellular vesicles

被引：57
作者：
Tian, Fei [1 ,4 ]
Liu, Chao [1 ,2 ,3 ]
Lin, Ling [1 ]
Chen, Qinghua [1 ]
Sun, Jiashu [1 ,5 ]
机构：
[1] Natl Ctr Nanosci & Technol, CAS Ctr Excellence Nanosci, CAS Key Lab Standardizat & Measurement Nanotechno, Beijing 100190, Peoples R China
[2] Southeast Univ, Sch Mech Engn, Nanjing 211189, Jiangsu, Peoples R China
[3] Southeast Univ, Jiangsu Key Lab Design & Manufacture Micronano Bi, Nanjing 211189, Jiangsu, Peoples R China
[4] Hebei Univ Technol, Sch Mech Engn, Tianjin 300401, Peoples R China
[5] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
来源：
TRAC-TRENDS IN ANALYTICAL CHEMISTRY | 2019年 / 117卷
基金：
中国国家自然科学基金; 中国博士后科学基金;
关键词：
Microfluidics; Circulating tumor cells (CTCs); Extracellular vesicles (EVs); Cancer diagnostics; Liquid biopsy; LABEL-FREE ISOLATION; RARE CANCER-CELLS; WHOLE-BLOOD; ELECTROCHEMICAL DETECTION; EFFICIENT ISOLATION; LUNG-CANCER; EXOSOMES; SEPARATION; CAPTURE; LIQUID;
D O I：
10.1016/j.trac.2019.05.013
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Liquid biopsy of circulating tumor cells (CTCs) and tumor-derived extracellular vesicles (EVs) holds immense promise in early detection of cancer, monitoring efficacy of therapy, and predicting prognosis. Microfluidic techniques provide new tools for isolation and detection of CTCs and EVs, by offering a multitude of advantages in terms of high-level of integration, multiplex analysis, small sample consumption, and point-of-care testing. This review summarizes recent advances in microfluidic isolation of CTCs and EVs using their physical and biological properties, and microfluidics-based detection of CTCs and EVs through a variety of sensing methods. Microfluidic analysis of single CTCs and EVs is highlighted to reveal their heterogeneity that is likely to be overlooked by conventional bulk methods. Challenges associated with microfluidics-based liquid biopsy of CTCs and EVs are discussed. (C) 2019 Elsevier B.V. All rights reserved.
引用
收藏
页码：128 / 145
页数：18
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