Getting More Out of Less - A Quantitative Serological Screening Tool for Simultaneous Detection of Multiple Influenza A Hemagglutinin-Types in Chickens

被引：17

作者：

Freidl, Gudrun S. ^{[1
,2
]}

de Bruin, Erwin ^{[2
]}

van Beek, Janko ^{[1
,2
]}

Reimerink, Johan ^{[2
]}

de Wit, Sjaak ^{[3
]}

Koch, Guus ^{[4
]}

Vervelde, Lonneke ^{[5
]}

van den Ham, Henk-Jan ^{[1
]}

Koopmans, Marion P. G. ^{[1
,2
]}

机构：

[1] Erasmus MC, Dept Virosci, Rotterdam, Netherlands

[2] Natl Inst Publ Hlth & Environm, RIVM, Ctr Infect Dis Control,Div Virol, Ctr Infect Dis Res Diagnost & Perinatal Screening, NL-3720 BA Bilthoven, Netherlands

[3] GD Anim Hlth Serv AHS, Deventer, Netherlands

[4] Cent Vet Inst, Lelystad, Netherlands

[5] Univ Utrecht, Utrecht, Netherlands

来源：

PLOS ONE | 2014年 / 9卷 / 09期

基金：

英国生物技术与生命科学研究理事会;

关键词：

CROSS-PROTECTION; VIRUS-INFECTION; ANTIBODIES; SUBTYPES; IMMUNOASSAY; RESPONSES; EPITOPE; H5;

D O I：

10.1371/journal.pone.0108043

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Current avian influenza surveillance in poultry primarily targets subtypes of interest for the veterinary sector (H5, H7). However, as virological and serological evidence suggest, surveillance of additional subtypes is important for public health as well as for the poultry industry. Therefore, we developed a protein microarray enabling simultaneous identification of antibodies directed against different HA-types of influenza A viruses in chickens. The assay successfully discriminated negative from experimentally and naturally infected, seropositive chickens. Sensitivity and specificity depended on the cutoff level used but ranged from 84.4% to 100% and 100%, respectively, for a cut off level of >= 1:40, showing minimal cross reactivity. As this testing platform is also validated for the use in humans, it constitutes a surveillance tool that can be applied in human-animal interface studies.

引用

页数：10

共 38 条

[1] SEQUENCE RELATIONSHIPS AMONG THE HEMAGGLUTININ GENES OF 12 SUBTYPES OF INFLUENZA-A VIRUS [J].