Development and evaluation of microsatellite markers in Phoenix dactylifera L. and their transferability to other Phoenix species

被引：36

作者：

Akkak, A. ^{[1
]}

Scariot, V. ^{[2
]}

Marinoni, D. Torello ^{[3
]}

Boccacci, P. ^{[3
]}

Beltramo, C. ^{[3
]}

Botta, R. ^{[3
]}

机构：

[1] Univ Foggia, Dipartimento Sci Agroambientali Chim & Difesa Veg, I-71100 Foggia, Italy

[2] Univ Turin, Dipartimento Agron Selvicoltura & Gest Territorio, I-10095 Grugliasco, Italy

[3] Univ Turin, Dipartimento Colture Arboree, I-10095 Grugliasco, Italy

来源：

BIOLOGIA PLANTARUM | 2009年 / 53卷 / 01期

关键词：

across-taxa transferability; date palm; simple sequence repeats; CULTIVARS;

D O I：

10.1007/s10535-009-0026-y

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Forty one simple sequence repeats were isolated from two microsatellite enriched libraries of date palm (Phoenix dactylifera L.). After screening, 17 selected microsatellite loci were characterized and evaluated on a set of 31 cultivars and clones from Algerian and Californian germplasm. All primer pairs produced an amplification product of the expected size and detected high polymorphism among the analysed samples. These nuclear simple sequence repeat (SSR) markers are expected to be a very effective tool for evaluating genetic diversity in date palm germplasm. Acrosstaxa amplification showed the usefulness of most SSR markers in 14 other species across the genus Phoenix.

引用

页码：164 / 166

页数：3

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