Epigallocatechin-3-Gallate Reduces Neuronal Apoptosis in Rats after Middle Cerebral Artery Occlusion Injury via PI3K/AKT/eNOS Signaling Pathway

被引：40

作者：

Wang Nan ^{[1
,2
]}

Xu Zhonghang ^{[1
]}

Chen Keyan ^{[3
]}

Liu Tongtong ^{[2
]}

Guo Wanshu ^{[2
]}

Xu Zhongxin ^{[1
]}

机构：

[1] Jilin Univ, China Japan Union Hosp, Dept Neurol, Changchun 130033, Jilin, Peoples R China

[2] Peoples Hosp Liaoning Prov, Dept Neurol, 33Wenyi Rd, Shenyang 110016, Liaoning, Peoples R China

[3] China Med Univ, Dept Lab Anim Sci, 77 Puhe Rd,Shenyang North New Area, Shenyang 110122, Liaoning, Peoples R China

来源：

BIOMED RESEARCH INTERNATIONAL | 2018年 / 2018卷

基金：

中国国家自然科学基金;

关键词：

GREEN TEA; (-)-EPIGALLOCATECHIN GALLATE; EGCG; ISCHEMIA; DAMAGE; REPERFUSION; ACTIVATION; INHIBITOR; STROKE; MODEL;

D O I：

10.1155/2018/6473580

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Background/Aims. Epigallocatechin-3-gallate (EGCG) has neuroprotective effects and the ability to resist amyloidosis. This study observed the protective effect of EGCG against neuronal injury in rat models of middle cerebral artery occlusion (MCAO) and investigated the mechanism of action of PI3K/AKT/eNOS signaling pathway. Methods. Rat models of permanent MCAO were established using the suture method. Rat behavior was measured using neurological deficit score. Pathology and apoptosis were measured using HE staining and TUNEL. Oxidative stress and brain injury markers were examined using ELISA. Apoptosis-related proteins and PI3K/AKT/eNOS signaling pathway were determined using western blot assay and immunohistochemistry. Results. EGCG decreased neurological function score, protected nerve cells, inhibited neuronal apoptosis, and inhibited oxidative stress injury and brain injury markers level after MCAO. EGCG reduced the apoptotic rate of neurons, increased the expression of Bcl-2, and decreased the expression of Caspase-3 and Bax. After LY294002 suppressed the PI3K pathway, the protective effect of EGCG decreased after administration of PI3K inhibitors. Conclusion. EGCG has a protective effect on rat brain injury induced by MCAO, possibly by modulating the PI3K/AKT/eNOS signaling pathway.

引用

页数：9