Epigallocatechin-3-Gallate Reduces Neuronal Apoptosis in Rats after Middle Cerebral Artery Occlusion Injury via PI3K/AKT/eNOS Signaling Pathway

被引：41

作者：

Wang Nan ^{[1
,2
]}

Xu Zhonghang ^{[1
]}

Chen Keyan ^{[3
]}

Liu Tongtong ^{[2
]}

Guo Wanshu ^{[2
]}

Xu Zhongxin ^{[1
]}

机构：

[1] Jilin Univ, China Japan Union Hosp, Dept Neurol, Changchun 130033, Jilin, Peoples R China

[2] Peoples Hosp Liaoning Prov, Dept Neurol, 33Wenyi Rd, Shenyang 110016, Liaoning, Peoples R China

[3] China Med Univ, Dept Lab Anim Sci, 77 Puhe Rd,Shenyang North New Area, Shenyang 110122, Liaoning, Peoples R China

来源：

BIOMED RESEARCH INTERNATIONAL | 2018年 / 2018卷

基金：

中国国家自然科学基金;

关键词：

GREEN TEA; (-)-EPIGALLOCATECHIN GALLATE; EGCG; ISCHEMIA; DAMAGE; REPERFUSION; ACTIVATION; INHIBITOR; STROKE; MODEL;

D O I：

10.1155/2018/6473580

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Background/Aims. Epigallocatechin-3-gallate (EGCG) has neuroprotective effects and the ability to resist amyloidosis. This study observed the protective effect of EGCG against neuronal injury in rat models of middle cerebral artery occlusion (MCAO) and investigated the mechanism of action of PI3K/AKT/eNOS signaling pathway. Methods. Rat models of permanent MCAO were established using the suture method. Rat behavior was measured using neurological deficit score. Pathology and apoptosis were measured using HE staining and TUNEL. Oxidative stress and brain injury markers were examined using ELISA. Apoptosis-related proteins and PI3K/AKT/eNOS signaling pathway were determined using western blot assay and immunohistochemistry. Results. EGCG decreased neurological function score, protected nerve cells, inhibited neuronal apoptosis, and inhibited oxidative stress injury and brain injury markers level after MCAO. EGCG reduced the apoptotic rate of neurons, increased the expression of Bcl-2, and decreased the expression of Caspase-3 and Bax. After LY294002 suppressed the PI3K pathway, the protective effect of EGCG decreased after administration of PI3K inhibitors. Conclusion. EGCG has a protective effect on rat brain injury induced by MCAO, possibly by modulating the PI3K/AKT/eNOS signaling pathway.

引用

页数：9

共 27 条

[1] RAT MIDDLE CEREBRAL-ARTERY OCCLUSION - EVALUATION OF THE MODEL AND DEVELOPMENT OF A NEUROLOGIC EXAMINATION [J].

BEDERSON, JB ;

PITTS, LH ;

TSUJI, M ;

NISHIMURA, MC ;

DAVIS, RL ;

BARTKOWSKI, H .

STROKE, 1986, 17 (03) :472-476

[2] The efficacy of Epigallocatechin-3-gallate (green tea) in the treatment of Alzheimer's disease: an overview of pre-clinical studies and translational perspectives in clinical practice [J].

Cascella, Marco ;

Bimonte, Sabrina ;

Muzio, Maria Rosaria ;

Schiavone, Vincenzo ;

Cuomo, Arturo .

INFECTIOUS AGENTS AND CANCER, 2017, 12

[3] Treatment with Enriched Environment Reduces Neuronal Apoptosis in the Periinfarct Cortex after Cerebral Ischemia/Reperfusion Injury [J].

Chen, Xiuping ;

Zhang, Xin ;

Xue, Li ;

Hao, Chizi ;

Liao, Weijing ;

Wan, Qi .

CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, 2017, 41 (04) :1445-1456

[4] Intranasal Administration of Interferon Beta Attenuates Neuronal Apoptosis via the JAK1/STAT3/BCL-2 Pathway in a Rat Model of Neonatal Hypoxic-Ischemic Encephalopathy [J].

Dixon, Brandon J. ;

Chen, Di ;

Zhang, Yang ;

Flores, Jerry ;

Malaguit, Jay ;

Nowrangi, Derek ;

Zhang, John H. ;

Tang, Jiping .

ASN NEURO, 2016, 8 (05)

[5] Synthetic lethality by co-targeting mitochondrial apoptosis and PI3K/Akt/mTOR signaling [J].

Fulda, Simone .

MITOCHONDRION, 2014, 19 :85-87

[6] Endothelial progenitor cells transplantation attenuated blood-brain barrier damage after ischemia in diabetic mice via HIF-1α [J].

Geng, Jieli ;

Wang, Liping ;

Qu, Meijie ;

Song, Yaying ;

Lin, Xiaojie ;

Chen, Yajing ;

Mamtilahun, Muyassar ;

Chen, Shengdi ;

Zhang, Zhijun ;

Wang, Yongting ;

Yang, Guo-Yuan .

STEM CELL RESEARCH & THERAPY, 2017, 8

[7] Long-term administration of green tea catechins improves spatial cognition learning ability in rats [J].

Haque, AM ;

Hashimoto, M ;

Katakura, M ;

Tanabe, Y ;

Hara, Y ;

Shido, O .

JOURNAL OF NUTRITION, 2006, 136 (04) :1043-1047

[8] Physiological effects of epigallocatechin-3-gallate (EGCG) on energy expenditure for prospective fat oxidation in humans: A systematic review and meta-analysis [J].

Kapoor, Mahendra P. ;

Sugita, Masaaki ;

Fukuzawa, Yoshitaka ;

Okubo, Tsutomu .

JOURNAL OF NUTRITIONAL BIOCHEMISTRY, 2017, 43 :1-10

[9] The antioxidant and pro-oxidant activities of green tea polyphenols: A role in cancer prevention [J].

Lambert, Joshua D. ;

Elias, Ryan J. .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2010, 501 (01) :65-72

[10] Protective effect of green tea polyphenol EGCG against neuronal damage and brain edema after unilateral cerebral ischemia in gerbils [J].

Lee, H ;

Bae, JH ;

Lee, SR .

JOURNAL OF NEUROSCIENCE RESEARCH, 2004, 77 (06) :892-900

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