Targeting cancer cells with biotin-dendrimer conjugates

被引：228
作者：
Yang, Wenjun [1 ,2 ]
Cheng, Yiyun [1 ,2 ]
Xu, Tongwen [3 ]
Wang, Xueyuan [1 ,2 ]
Wen, Long-ping [1 ,2 ]
机构：
[1] Univ Sci & Technol China, Hefei Natl Lab Phys Sci Microscale, Hefei 230027, Anhui, Peoples R China
[2] Univ Sci & Technol China, Sch Life Sci, Hefei 230027, Anhui, Peoples R China
[3] Univ Sci & Technol China, Dept Chem, Lab Funct Membranes, Hefei 230026, Anhui, Peoples R China
来源：
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY | 2009年 / 44卷 / 02期
关键词：
Dendrimer; Cancer targeting; Biotin; Conjugate; Nano-carrier; DEPENDENT MULTIVITAMIN TRANSPORTER; NEUTRON-CAPTURE THERAPY; EPIDERMAL-GROWTH-FACTOR; DRUG-DELIVERY; POLYMER; ARCHITECTURE; PRODRUGS; CARRIERS;
D O I：
10.1016/j.ejmech.2008.04.021
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
Star-burst dendrimers represent a superior carrier platform for targeted drug delivery. Partially acetylated generation 5 (G5) polyamidoamine (PAMAM) dendrimer was conjugated with the targeting moiety (biotin) and the imaging moiety (fluoresceinisothiocyanate, FITC), and the resulting dendrimer-biotin conjugate was characterized by H-1 NMR, UV-vis spectrum. As revealed by flow cytometry and confocal microscopy, the bifunctional conjugate (dendrimer-biotin-FITC) exhibited much higher cellular uptake into HeLa cells than the conjugate without biotin. The uptake was energy-dependent, dose-dependent, and could be effectively blocked by dendrimer-conjugated biotin. Our results indicated that the biocompatible biotin-dendrimer conjugate might be a promising nano-platform for cancer therapy and cancer diagnosis. (c) 2008 Elsevier Masson SAS. All rights reserved.
引用
收藏
页码：862 / 868
页数：7
相关论文
共 44 条
[1]   Poly(amidoamine) (PAMAM) dendritic nanostructures for controlled site-specific delivery of acidic anti-inflammatory active ingredient [J].
Asthana, A ;
Chauhan, AS ;
Diwan, PV ;
Jain, NK .
AAPS PHARMSCITECH, 2005, 6 (03)
[2]   Neutron capture therapy of epidermal growth factor (plus) gliomas using boronated cetuximab (IMC-C225) as a delivery agent [J].
Barth, RF ;
Wu, G ;
Yang, WL ;
Binns, PJ ;
Riley, KJ ;
Patel, H ;
Coderre, JA ;
Tjarks, W ;
Bandyopadhyaya, AK ;
Thirumamagal, BTS ;
Ciesielski, MJ ;
Fenstermaker, RA .
APPLIED RADIATION AND ISOTOPES, 2004, 61 (05) :899-903
[3]  
Bhadra D, 2005, J PHARM PHARM SCI, V8, P467
[4]   Glycodendrimeric nanoparticulate carriers of primaquine phosphate for liver targeting [J].
Bhadra, D ;
Yadav, AK ;
Bhadra, S ;
Jain, NK .
INTERNATIONAL JOURNAL OF PHARMACEUTICS, 2005, 295 (1-2) :221-233
[5]   A PEGylated dendritic nanoparticulate carrier of fluorouracil [J].
Bhadra, D ;
Bhadra, S ;
Jain, S ;
Jain, NK .
INTERNATIONAL JOURNAL OF PHARMACEUTICS, 2003, 257 (1-2) :111-124
[6]   Dendrimers in drug research [J].
Boas, U ;
Heegaard, PMH .
CHEMICAL SOCIETY REVIEWS, 2004, 33 (01) :43-63
[7]  
Cannizzaro SM, 1998, BIOTECHNOL BIOENG, V58, P529, DOI 10.1002/(SICI)1097-0290(19980605)58:5<529::AID-BIT9>3.3.CO
[8]  
2-U
[9]   Pharmaceutical applications of dendrimers: promising nanocarriers for drug delivery [J].
Cheng, Yiyun ;
Wang, Jinrong ;
Rao, Tingling ;
He, Xiaoxiao ;
Xu, Tongwen .
FRONTIERS IN BIOSCIENCE-LANDMARK, 2008, 13 :1447-1471
[10]   Dendrimers as drug carriers: Applications in different routes of drug administration [J].
Cheng, Yiyun ;
Xu, Zhenhua ;
Ma, Minglu ;
Xu, Tongwen .
JOURNAL OF PHARMACEUTICAL SCIENCES, 2008, 97 (01) :123-143
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