Unusually Broad Substrate Profile of Self-Sufficient Cytochrome P450 Monooxygenase CYP116B4 from Labrenzia aggregata

被引：65
作者：
Yin, Yue-Cai [1 ]
Yu, Hui-Lei [1 ,2 ]
Luan, Zheng-Jiao [1 ]
Li, Ren-Jie [1 ]
Ouyang, Peng-Fei [1 ]
Liu, Jing [1 ]
Xu, Jian-He [1 ,2 ]
机构：
[1] E China Univ Sci & Technol, Lab Biocatalysis & Synthet Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China
[2] Shanghai Collaborat Innovat Ctr Biomfg, Shanghai 200237, Peoples R China
来源：
CHEMBIOCHEM | 2014年 / 15卷 / 16期
基金：
中国国家自然科学基金;
关键词：
cytochromes; data mining; hydroxylation; oxidoreductases; protein engineering; substrate promiscuity; ENANTIOSELECTIVE SYNTHESIS; LIVER MICROSOMES; RHODOCOCCUS SP; STEREOSELECTIVITY; HYDROXYLATION; EVOLUTION; ALCOHOLS;
D O I：
10.1002/cbic.201402309
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A new member of the CYP116B subfamilyP450(LaMO)was discovered in Labrenzia aggregata by genomic data mining. It was successfully overexpressed in Escherichia coli, purified, and subsequently characterized spectroscopically, and its catalytic properties were assessed. Substrate profiling of the P450(LaMO) revealed that it was a versatile catalyst, exhibiting hydroxylation and epoxidation activities as well as O-dealkylation and asymmetric sulfoxidation activities. Diverse compounds, including alkylbenzenes, aromatic bicyclic molecules, and terpenoids, were shown to be hydroxylated by P450(LaMO). Such diverse catalytic activities are uncommon for the bacterial P450s, and the P450(LaMO) -mediated stereoselective hydroxylation of inactivated CH bondsubiquitous and relatively unreactive in organic moleculesis particularly unusual. The self-sufficient nature of P450(LaMO), coupled with its broad substrate range, highlights it as an ideal template for directed evolution towards various applications.
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收藏
页码：2443 / 2449
页数：7
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